Research Article
Neferine Protects against Hypoxic-Ischemic Brain Damage in Neonatal Rats by Suppressing NLRP3-Mediated Inflammasome Activation
Figure 6
Silencing NLRP3 could enhance the inhibitory effect of neferine on CoCl2-induced PC12 pyroptosis and oxidative stress. (a) Mitochondrial ROS generation of each group was detected by MitoSox (red) and DAPI (blue) staining. (b, c) Intracellular ROS was analyzed using flow cytometry after DCF-DA staining. , , and . . (d) The GSH-px level in PC12. , , and . . (e) The SOD level in PC12. and . . (f) The protein levels of cleaved caspase-1, GSDMD, ASC, NLRP3, IL-1β, and IL-18 were evaluated by Western blotting in PC12. (g–l) Analyses of cleaved caspase-1, GSDMD, ASC, NLRP3, IL-1β, and IL-18 (of β-actin). , , and . . (m, n) Representative immunofluorescence staining images of NLRP3 and cleaved caspase-1 (red) in PC12. The nucleus (blue) was labeled with DAPI. Scale .
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