Resveratrol Alleviates 27-Hydroxycholesterol-Induced Senescence in Nerve Cells and Affects Zebrafish Locomotor Behavior via Activation of SIRT1-Mediated STAT3 Signaling

Figure 3

The 27HC downregulates SIRT1 expression via ROS-mediated methylation. BV2 and PC12 cells were treated with 10 μM 27HC, 100 μM SAM, NAC (pretreatment for 2 h), and (pretreatment for 2 h) for 12 h; CON group: ethanol treatment. (a, c) Western blot analysis of DNMT1, SIRT1, and β-actin. (b, d) The quantitative analysis of protein bands of (a, c). , statistically significant difference from the CON group. (b) BV2: (DNMT1: , 27HC group; , SAM group; SIRT1: , 27HC group; , SAM group). (d) PC12: (DNMT1: , 27HC group; , SAM group; SIRT1: , 27HC group; , SAM group). ^#, statistically significant difference from the 27HC-treated group. (b) BV2: (DNMT1: , 27HC+NAC group; , 27HC+AZA group; SIRT1: , 27HC+NAC group; , 27HC+AZA group). (d) PC12: (DNMT1: , 27HC+NAC group; , 27HC+AZA group; SIRT1: , 27HC+NAC group; , 27HC+AZA group). (e, f) qRT-PCR analysis of SIRT1. , statistically significant difference from the CON group. (e) BV2: , 27HC group; , SAM group. (f) PC12: , 27HC group; , SAM group. ^#, statistically significant difference from the 27HC-treated group. (e) BV2: , 27HC+NAC group; , 27HC+AZA group. (f) PC12: , 27HC+NAC group; , 27HC+AZA group. Data are expressed as the of three independent experiments.

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