Chinese Herbal Prescription Fu-Zheng-Qu-Xie Prevents Recurrence and Metastasis of Postoperative Early-Stage Lung Adenocarcinoma: A Prospective Cohort Study Followed with Potential Mechanism Exploration

Figure 2

The FZQX prescription inhibited the growth of subcutaneous xenograft of LLC cells in vivo. (a) Representative photographs of xenograft tumors were taken 21 days at the experimental endpoint. (b) Weight of the xenograft tumors harvested from mice in each group. (c) The volume of the xenograft tumors harvested from mice in each group. (d) Dynamic change in the bodyweight of mice in each group. (e) Expression levels of proteins associated with MDSC immunosuppression function and tumor progression in each group detected by Western blot. (f) Expression levels of MMP9, pNF-κB, and IL-1β proteins in the tumor tissues analyzed by immunohistochemistry. (g) Densitometric analysis of Western blot. (h) The signal intensity of immunofluorescent images quantified by ImageJ software. NS: the normal saline group (control); F: the FZQX prescription group; GEM: the gemcitabine group; G+F: the gemcitabine plus FZQX group. All data are expressed as the from at least three independent experiments. ns: no significant difference, , , , . The same as below.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)