Research Article

Polydatin Attenuates OGD/R-Induced Neuronal Injury and Spinal Cord Ischemia/Reperfusion Injury by Protecting Mitochondrial Function via Nrf2/ARE Signaling Pathway

Figure 6

PD-mediated mito-protective depends on the Nrf2/ARE pathway. (a) [Ca2+]i levels in cells exposed to normoxia or hypoxia-reperfusion in the indicated groups. (b) NRFU of calcein indicating mPTP opening in the differentially treated cells. (c) The ratios of polymeric (red) and monomeric (green) forms of JC-1 corresponding to the MMP in the indicated groups. (d) MitoSOX fluorescence intensity indicative of ROS levels. Data are presented as fold change over the Control group. μm. (e) Neuronal ATP release (nmol/mg protein) as measured by a luciferase-based assay. vs. Control; # vs. OGD; Δ vs. OGD + PD.
(a)
(b)
(c)
(d)
(e)