Pentoxifylline Enhances Antioxidative Capability and Promotes Mitochondrial Biogenesis in D-Galactose-Induced Aging Mice by Increasing Nrf2 and PGC-1α through the cAMP-CREB Pathway

Table 1

qPCR primer sequences.

Gene	Forward primer (5-3)	Reverse primer (5-3)
For mouse genes
Senescence
p16	GAACTCTTTCGGTCGTACCC	CGAATCTGCACCGTAGTTGA
Ager	ACTACCGAGTCCGAGTCTACC	CCCACCTTATTAGGGACACTGG
Oxidative stress
HO-1	CAACGAATCTTGAATGGAGG	AGGTGTCCAGAGAAGGCTT
NQO1	ATCCTTCCGAGTCATCTCTA	CAACGAATCTTGAATGGAGG
SOD2	TGAACAACCTCAACGCCAC	GAAGGTAGTAAGCGTGCTC
CAT	AACTGGGATCTTGTGGGAA	GACAGTTCACAGGTATCTG
GPx1	CTCACCCGCTCTTTACCTTCCT	ACACCGGAGACCAAATGATGTACT
Mitochondrial biogenesis
PGC-1α	GAAAGGGCCAAACAGAGAGA	GTAAATCACACGGCGCTCTT
NRF-1	TGGAGTCCAAGATGCTAATG	AGAGCTCCATGCTACTGTTC
TFAM	CAGGAGGCA AAGGATGATTC	CCA AGACTTCATTTCATTGTCG
Housekeeping gene
GAPDH	ACTCTTCCACCTTCGATGCC	TCTTGCTCAGTGTCCTTGCT
For human genes
CREB	ATCTTAGTGCCCAGCAACCA	ACATGTTACCATCTTCAAACTGACG
GAPDH	AGAAGGCTGGGGCTCATTTG	AGGGGCCATCCACAGTCTTC