Increased ROS-Dependent Fission of Mitochondria Causes Abnormal Morphology of the Cell Powerhouses in a Murine Model of Amyotrophic Lateral Sclerosis

Figure 1

Disturbed mitochondrial network in wobbler α-motor neurons. (a) Motor neuron enriched cultures of p40 WT and WR after 10d in vitro. Staining was performed with CellTracker (green) and MitoTracker (red). The mitochondrial network was reconstructed and quantitatively analyzed with Imaris 9.2.1 (b) spots and (c) surface function. Due to a significant decrease in area, volume, and diameter of spots as well a slightly diminished surface area and volume, a disturbed, fragmented mitochondrial network is present in wobbler motor neurons. No clear differences could be found in surface ellipsoid axes. Mean and maximum MitoTracker intensity of spots and surfaces are significantly higher in wobbler motor neurons, probably explained by more individual, smaller mitochondria per area. Data are presented as means ± SEM. For significance testing, students t-test was performed. Significant differences are indicated by , , , and . (a). A total of 50 motoneurons from four independent preparations per genotype were examined. -11815; -1433.

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