Vitamin B₁₂ Attenuates Acute Pancreatitis by Suppressing Oxidative Stress and Improving Mitochondria Dysfunction via CBS/SIRT1 Pathway

Figure 1

VB 12 improves pancreatic damage in acute pancreatitis by activating CBS. Animals were randomly divided into four experimental groups: AP group treated with NaT (3%), NaT + VB 12 (20 μg/kg, ip), NaT + VB 12 (40 μg/kg, ip) and control group treated with 0.9% saline (). NaT (3%) was injected through the pancreaticobiliary tract, and pancreatic tissue and blood were collected at 24 hours for follow-up studies. (a) The expression of CBS in pancreatic tissue was measured by Western blot. (b) The level of Hcy in serum was detected by ELISA assay kit. (c) The GSH/GSSG ratio in pancreatic tissue was detected by assay kit. (d, e, g) The levels of serum amylase, lipase, and pancreatic myeloperoxidase activity were detected. (f) Representative histopathological sections of pancreatic tissues by hematoxylin-eosin staining. Scale bar: 50 μm. (h) The histological scores of pancreatic edema, inflammatory infiltration, and necrosis were obtained to evaluate the degree of injury. Slides were evaluated by two pathologists in a blinded manner. The results are presented as the . , , vs. Con group. ^#, ^##, ^### vs. NaT group. ^∆, ^∆∆, ^∆∆∆ vs. VB 12(20 μg/kg) treatment group. Con: control group; NaT: acute pancreatitis group.

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