Research Article

TNF-α Induces Neutrophil Apoptosis Delay and Promotes Intestinal Ischemia-Reperfusion-Induced Lung Injury through Activating JNK/FoxO3a Pathway

Figure 6

ABT-199 promotes polymorphonuclear neutrophil (PMN) apoptosis to alleviate intestinal ischemia-reperfusion- (IIR-) induced lung injury. A mouse model of IIR injury was constructed and treated with Bcl-2 inhibitor ABT-199, followed by using immunohistochemical staining to detect the infiltration of Ly6G-positive PMNs in the lung tissue (a) and hematoxylin and eosin (HE) staining to detect the pathomorphological changes in the lung tissue (b). The extent of lung injury was assessed by Koksel’s scoring method (c), and the water content of lung tissue was also determined (d). Each bar represents the ( per group). , , one-way ANOVA with Tukey test. Sham: sham-operated group; IIR: intestinal ischemia-reperfusion model group; ABT-199: intravenous administration of ABT-199 (10 mg/kg) alone; ABT-199+IIR group: immediate administration of ABT-199 (10 mg/kg) at the onset of IIR.
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