Low-Grade Systemic Inflammation Interferes with Anabolic and Catabolic Characteristics of the Aged Human Skeletal Muscle

Figure 4

The increased proteasome activity at basal state coincides with increased protein carbonylation and IKKα/β phosphorylation. (a) Quantification of protein carbonyl groups (OxyBlot) by densitometry (results are expressed as ratio of protein carbonyl groups to GAPDH) and representative western blots (9 in ESI and 11 in control). (b) Quantification of mean fluorescence intensity for 3-nytrotirosine and (c) representative images of 3-nitrotyrosine localization in skeletal muscle (i: 3-nitrotyrosine/DAPI stain of a muscle cross section; ii and iii: single channel views of 3-nitrotyrosine and DAPI) ( in ESI and 10 in control). (d) Quantification of mean fluorescence intensity for phosphorylated IKKα/β and (e) representative images of phosphorylated IKKα/β localization in skeletal muscle (i: phosphorylated IKKα/β/DAPI stain of a muscle cross section; ii and iii: single channel views of phosphorylated IKKα/β and DAPI) ( in ESI and 10 in control). (f) Quantification of mean fluorescence intensity for Nrf2 into the nulcei and (g) representative images of Nrf2 localization in skeletal muscle (i: Nrf2/DAPI stain of a muscle cross section; ii and iii: single channel views of Nrf2 and DAPI) ( in ESI and 9 in Control). Significant difference from basal (). ^#Significant difference between groups (). All data are presented as .

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