MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer’s Disease

Figure 2

Immunohistochemical detection of MUTYH protein in the human brain. (a–d) Control (non-AD) brain and (e–h) AD brain. (a, e) Low-power view of hippocampal formation and parahippocampal gyrus. MUTYH immunoreactivities were detected in the GCL of the DG and in the pyramidal cell layers of the CA1-CA4 fields and subicular, entorhinal, and perirhinal cortices in both non-AD and AD brains. Nuclei were counterstained by hematoxylin. (b, f) Pyramidal cells in the CA1 field exhibit granular and diffuse MUTYH immunoreactivities in the cytoplasm (i) and to a lesser extent in the nucleus (ii). Intense immunoreactivities were evident in the perinuclear area. (c, g) Glial cells (iii) in the CA1 field exhibit weak MUTYH immunoreactivities in both the nucleus and cytoplasm. (d, h) Granule cells in DG also exhibit granular and diffuse MUTYH immunoreactivities in the cytoplasm (i) and to a lesser extent in the nucleus (ii). Scale bars: 1 mm (a, e) and 20 μm (b–d, f–h). (i) Intensity of MUTYH immunoreactivities (MUTYH IR) in the CA1 field and DG. The data are shown as boxplots with dots and whiskers indicating the minimum and maximum (). Statistical analyses were performed with Wilcoxon’s rank sum test, and the value is shown in each graph. Similar levels of MUTYH immunoreactivities were detected in both non-AD and AD hippocampi.

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