Oxidative Medicine and Cellular Longevity

Research Article

MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer’s Disease

Table 3

The altered expression of microglial stage-specific genes in the hippocampi of 6-month-old female wild-type, App^{NL-G-F/NL-G-F}, and App^{NL-G-F/NL-G-F}·Mutyh^-/- mice.


Gene	Average expression (log2)			-test	App^{NL-G-F/NL-G-F} vs. wild type		App^{NL-G-F/NL-G-F} ·Mutyh^-/- vs. App^{NL-G-F/NL-G-F}		App^{NL-G-F/NL-G-F} ·Mutyh^-/- vs. wild type		Cell
Symbol^b	Wild type	App^{NL-G-F/NL-G-F}	App^{NL-G-F/NL-G-F}·Mutyh^-/-	value^a	Fold change	value^a	Fold change	value^a	Fold change	value^a	Type

Hexb	9.55	10.14	10.17		1.50		1.02	0.7434	1.53		Homeostatic
C1qb	9.33	9.87	10.01		1.45		1.10	0.4371	1.60
Ctss	9.62	10.33	10.48		1.65	0.0001	1.11	0.4902	1.82
C1qc	7.08	7.72	7.62	0.0004	1.56	0.0002	-1.07	0.1922	1.46	0.001
Csf1r	8.66	8.97	9.03	0.0006	1.24	0.0006	1.04	0.7342	1.29	0.0004
Olfml3	7.13	7.50	7.38	0.003	1.29	0.001	-1.08	0.1532	1.19	0.0101
C1qa	7.99	8.66	8.91	0.0042	1.60	0.0034	1.18	0.9048	1.89	0.0028
*Sparc*	9.57	9.77	9.46	0.0422	1.15	0.0919	-1.24	0.015	-1.08	0.2911
Tmsb4x	7.91	8.00	8.07	0.0917	1.06	0.1379	1.05	0.4195	1.11	0.0357
Cx3cr1	8.22	8.38	8.31	0.1034	1.12	0.0383	-1.05	0.2926	1.06	0.2214
P2ry12	7.92	8.00	8.09	0.163	1.06	0.8204	1.06	0.085	1.12	0.1228
Cst3	11.42	11.51	11.36	0.3314	1.06	0.1883	-1.11	0.2231	-1.05	0.9107

Tyrobp	6.61	7.37	7.17		1.69		-1.14	0.1519	1.47		Stage 1 DAM
*Ctsd*	10.90	11.60	11.31	0.0003	1.63		-1.23	0.0034	1.33	0.0191
*Lyz2*	5.96	7.10	6.67	0.0013	2.21	0.0004	-1.35	0.0314	1.64	0.0142
B2m	11.24	11.57	11.68	0.0335	1.26	0.0475	1.08	0.4576	1.36	0.0136
Ctsb	11.65	11.76	11.81	0.0677	1.07	0.053	1.04	0.8195	1.11	0.0362
*Timp2*	10.13	10.11	9.25	0.0753	-1.01	0.7337	-1.82	0.0371	-1.84	0.0655
Cstb	7.43	7.57	7.34	0.1574	1.10	0.2916	-1.18	0.0614	-1.07	0.3341
H2-D1	5.54	5.71	5.85	0.3992	1.13	0.1978	1.11	0.6835	1.24	0.357
Apoe	7.82	7.89	7.81	0.5273	1.05	0.4561	-1.06	0.2807	-1.01	0.7201
Fth1	13.00	13.03	13.03	0.7389	1.02	0.4795	1.00	0.9052	1.02	0.5536

*Cd68*	7.90	8.88	8.68		1.97		-1.15	0.0048	1.71		Stage 2 DAM
Clec7a	4.53	6.36	6.52		3.56		1.11	0.3111	3.97
*Cst7*	5.53	7.64	6.95		4.33		-1.61	0.0089	2.69
*Trem2*	7.01	7.73	7.50		1.65		-1.17	0.0246	1.41
Itgax	5.13	5.59	5.79		1.37	0.0002	1.15	0.0709	1.58
*Ccl6*	4.78	5.90	5.46	0.0003	2.18		-1.36	0.0312	1.61	0.0023
Cd52	5.77	6.36	6.33	0.0017	1.51	0.0007	-1.02	0.2591	1.48	0.0037
Gusb	7.07	7.34	7.36	0.0087	1.21	0.0049	1.01	0.7505	1.22	0.0081
Ctsz	9.08	9.55	9.39	0.0108	1.38	0.0037	-1.12	0.2169	1.24	0.0301
Cd9	8.59	8.96	8.84	0.0441	1.29	0.0293	-1.08	0.982	1.19	0.0282
Cd63	8.48	8.90	8.61	0.0745	1.34	0.0289	-1.22	0.4213	1.09	0.1122
Ctsa	9.29	9.41	9.37	0.1195	1.09	0.0615	-1.03	0.7992	1.06	0.0935
Serpine2	9.45	9.59	9.47	0.1448	1.10	0.056	-1.08	0.3433	1.02	0.2617
Cadm1	9.57	9.44	9.11	0.1544	-1.09	0.8814	-1.26	0.1083	-1.38	0.085
Csf1	7.39	7.53	7.57	0.2612	1.10	0.1746	1.03	0.9153	1.13	0.1479
Axl	8.11	8.19	8.17	0.2701	1.06	0.1175	-1.02	0.5009	1.04	0.3287
Ctsl	8.47	8.62	8.50	0.2709	1.11	0.1201	-1.09	0.2994	1.02	0.5517
Spp1	5.99	5.97	5.99	0.294	-1.01	0.1361	1.02	0.6252	1.00	0.2865
Lpl	7.29	7.33	7.25	0.3032	1.03	0.3701	-1.05	0.1342	-1.02	0.4987
Ank	9.54	9.55	9.61	0.7864	1.00	0.9266	1.04	0.5923	1.04	0.5321
Hif1a	8.67	8.69	8.69	0.8123	1.01	0.5354	1.00	0.8183	1.01	0.6928

^a values < 0.05 are indicated in bold. ^bGenes whose expression differed significantly altered between App^{NL-G-F/NL-G-F} and App^{NL-G-F/NL-G-F}·Mutyh^-/- are shown in bold. DAM=disease-associated microglia.