Inhibition of Growth of Colon Tumors and Proliferation of HT-29 Cells by Warburgia ugandensis Extract through Mediating G₀/G₁ Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation

Figure 5

Apoptosis assays in CRC cells. HT-29 (a, b, c) and HCT116 cells (e, f, g) were treated with WUD for 24 h, then the cells were co-stained with Annexin V-FITC/PI and subjected to fluorescence microscopy and flow cytometry analysis. (d, h) Protein levels of uncleaved PARP1, cleaved PARP1, and pro-caspase 3 were assessed with western blot after being incubated with WUD for 24 h. β-Actin was used as a loading control. Data were . . ns: not significant.

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