hsa-miR-199b-3p Prevents the Epithelial-Mesenchymal Transition and Dysfunction of the Renal Tubule by Regulating E-cadherin through Targeting KDM6A in Diabetic Nephropathy

Figure 4

Target prediction and dual-luciferase assay between miRNAs and KDM6A. (a) The relative level of miRNA expression by qPCR in HK2 cells cultured in HG or Mann for 72 h. . (b) The relative expression level of miRNAs by qPCR in kidneys from C57BL/Ks mice (WT-NC) and STZ-induced mice (WT-STZ) (at 8 weeks after the onset of diabetes). . (c) Schematic diagram of target prediction between KDM6A and hsa-miR-199b-3p. (d) Regulation of hsa-miR-199b-3p on 3-UTR of KDM6A in HEK293T cells by the luciferase reporter assay. . values is presented. , , and (Student’s -test).

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