TY - JOUR A2 - Sinha, Mithun AU - Hua, Hai-Xin AU - Deng, Hai-Bo AU - Huang, Xiu-Ling AU - Ma, Chang-Qing AU - Xu, Ping AU - Cai, Ye-Hua AU - Wang, Hai-Tang PY - 2021 DA - 2021/01/20 TI - Effects of Occupational Exposure to Waste Anesthetic Gas on Oxidative Stress and DNA Damage SP - 8831535 VL - 2021 AB - Objective. The aim of the study was to investigate the potential effects of waste anesthetic gas (WAG) on oxidative stress, DNA damage, and vital organs. Methods. A total of 150 members of the staff at a hospital were assigned to an exposure group or control group. The 68 operating room (OR) staff in the exposure group were exposed to WAG, and the 82 non-OR staff in the control group were not exposed to WAG. Air samples were collected in the OR, and the sevoflurane concentrations in the samples were determined. Superoxide dismutase (SOD), glutathione peroxidase (GSH-px), and malondialdehyde (MDA) in plasma from the participants were determined to assess oxidative stress. Western blot analysis was used to detect γH2AX in peripheral blood to assess DNA damage. Hematopoietic parameters, liver function, kidney function, and changes in electrophysiology were assessed to identify the effects on the vital organs. Results. The mean (±standard deviation) sevoflurane concentration in 172 air samples from 22 operating rooms was 1.11±0.65ppm. The superoxide dismutase activity and vital organ parameters (lymphocyte, hemoglobin, and total protein concentrations and heart rate) were significantly lower (P<0.05) in the exposed group than the control group. The malondialdehyde, total bilirubin, and creatinine concentrations and QT and QTc intervals were significantly higher (P<0.05) in the exposed group than the control group. There were no significant differences between the glutathione peroxidase activities and γH2AX concentrations for the exposed and control groups. Conclusions. Long-term occupational exposure to waste anesthetic gas may affect the antioxidant defense system and probably affects vital organ functions to some extent. No correlation between DNA damage and chronic exposure to WAG was observed. SN - 1942-0900 UR - https://doi.org/10.1155/2021/8831535 DO - 10.1155/2021/8831535 JF - Oxidative Medicine and Cellular Longevity PB - Hindawi KW - ER -