The Activation of ROS/NF-κB/MMP-9 Pathway Promotes Calcium-Induced Kidney Crystal Deposition

Figure 3

Effects of high concentration of calcium on the expressions of NF-κB signaling pathway proteins and osteoblastic-related proteins in NRK-52E cells. (a) The expressions of OPN, RUNX2, and E-cadherin mRNAs were detected by q-PCR assay after treatment with 0.7 mg/ml and 1.0 mg/ml calcium for 24 hours. (b) The expressions of OPN, RUNX2, and E-cadherin proteins were detected by western blot assay after treatment with 0.7 mg/ml and 1.0 mg/ml calcium for 24 hours. Quantitative analysis of the densities of western blot bands. (c) The expressions of NF-κB signaling pathway proteins were detected by western blot assay after treatment with 0.7 mg/ml and 1.0 mg/ml calcium for 24 hours. Quantitative analysis of the densities of western blot bands. (d) Intracellular p-p65 distribution was detected by immunofluorescence assay after treatment with 0.7 mg/ml and 1.0 mg/ml calcium for 24 hours (magnification 1000x). versus the control group, versus the control group.

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