Research Article
The Activation of ROS/NF-κB/MMP-9 Pathway Promotes Calcium-Induced Kidney Crystal Deposition
Figure 7
Effects of MMP-9 inhibitor I on calcium crystal deposition and osteogenic related proteins expression in 1,25(OH)2D3-induced SD rat kidney. (a) von Kossa staining detected calcium crystal deposition at the junction of renal cortex and medulla in 1,25(OH)2D3-induced SD rat kidney (Aa–d, magnification 100x; Ae–h, magnification 200x). Quantification of calcium crystal deposition level. (b) The expressions of MMP-9, OPN, RUNX2, and E-cadherin in rat kidney tissues were detected by immunohistochemical staining (magnification 400x). Quantification of immunohistochemical staining for MMP-9, OPN, RUNX2, and E-cadherin by Image-Pro Plus software. (c) The expressions of MMP-9, OPN, RUNX2, and E-cadherin mRNAs were detected by q-PCR assay in rat kidney tissues. (d) The expressions of MMP-9, OPN, RUNX2, and E-cadherin proteins were detected by western blot assay in rat kidney tissues. Quantitative analysis of the densities of western blot bands. (e) Immunofluorescence double-standard staining was used to detect the expression differences of RUNX2 and E-cadherin. Quantification of the mean fluorescence values of RUNX2 and E-cadherin in rat renal tissue. versus the control group, versus the control group.
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