Sea Cucumber-Derived Peptides Alleviate Oxidative Stress in Neuroblastoma Cells and Improve Survival in C. elegans Exposed to Neurotoxic Paraquat

Figure 5

Cellular uptake of rhodamine B-labelled peptides. (a) Confocal imaging of SH-SY5Y cells incubated with either 2 μM or 50 μM rhodamine B-labelled peptide TP-WW-623 for 5 to 30 min at 37°C to show the cellular uptake mechanism. The ROI (region of interest) panel represents zoomed images from the marked squares in the panels labelled “rhodamine B.” (b) SH-SY5Y cells were incubated at 4°C for 15 min to block endocytosis before incubating them with either rhodamine B-labelled TP-WW-623 or free rhodamine B for a further 10 min at room temperature. These cells were then washed three times with PBS before imaging. Experiments were repeated twice.

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