Velvet Antler Methanol Extracts Ameliorate Parkinson’s Disease by Inhibiting Oxidative Stress and Neuroinflammation: From C. elegans to Mice

Figure 3

MEs inhibit ROS generation in the activated microglia. (a) The endpoint microscope fluorescence image of intracellular ROS of BV2 cells. Intracellular ROS generation was measured by fluorescence staining with H₂DCF-DA. LPS (200 ng/mL)-activated BV2 cells were treated with MEs (20 and 40 μg/mL), scale  μm. The quantitative analysis of the fluorescence intensity of DCF shown in (a). (b) TUNEL staining. LPS (200 ng/mL)-activated BV2 cells were treated with MEs (20 and 40 μg/mL) for 24 h. DNA fragmentation was detected by TUNEL assay. Scale  μm.

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