Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates

Figure 7

The direct effects of BK on prostate cells. (a) The BDKRB2 (red) expression in WPMY-1 and RPrPF (magnification ×200). (b, c) MTT results of WPMY-1 and RPrPF under administration of BK in different concentrations. Results were plotted as OD values. , (WPMY-1 + BK vs. the vehicle group at the same time point in 3 independent repeated experiment). ^#, ^## (RPrPF + BK vs. the vehicle group at the same time point in 3 independent repeated experiment). (d) Relative mRNA expression of the TGF-β1 and iNOS to β-actin within WPMY-1 and RPrPF under 10 nM BK by RT-PCR. (e, f) TGF-β1 and iNOS protein expression within WPMY-1 and RPrPF under 10 nM BK by Western blot. Each bar represents of 3 independent repeated experiment. ^§§ (WPMY-1 + BK vs. WPMY-1 + vehicle). ^$$ (RPrPF + BK vs. RPrPF + vehicle).

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