Hydrogen Sulfide Is a Regulator of Hemoglobin Oxygen-Carrying Capacity via Controlling 2,3-BPG Production in Erythrocytes

Figure 8

Reduced H₂S production contributes to hypoxia-induced increased 2,3-BPG and P50 levels and translocation of BPGM and Hb between the membrane and the cytosol in mouse RBCs. (a–c) The effects of hypoxia on 2,3-BPG, P50, and H₂S levels. WT mice were exposed to hypoxia (10% oxygen) for the indicated time. Blood samples were collected for the determination of erythrocyte 2,3-BPG (a) and P50 (b) levels, as well as the H₂S level in plasma (c). Data are expressed as (). , . (d–h) The effects of GYY4137 on hypoxia-induced elevated 2,3-BPG and P50 levels and translocation of BPGM and Hb from the membrane to the cytosol. WT mice were exposed to hypoxia (10% oxygen) with the administration of GYY4137 or saline. After hypoxia and treatment of GYY413 for 24 h, the blood samples were collected for determination of erythrocyte 2,3-BPG (d), P50 (e), membrane heme concentration (f), cytosolic BPGM activity (g), and membrane and cytosolic BPGM levels (h). Data are expressed as (). . (h) Representative images of S-sulfhydration of Hb in erythrocytes of the mice. WT mice were exposed to hypoxia (10% oxygen) with the administration of GYY4137 (50 mg/kg) or saline. After hypoxia and treatment of GYY413 for 24 h, the blood samples were collected for determination of S-sulfhydration of Hb using the biotin-switch technique.

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