Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats

Figure 2

Time course of albumin and HO-1 expression as well as cellular localization of neurons after ICH. (a) Representative WB bands and (b, c) quantitative analysis of albumin and HO-1 expression after ICH; vs. sham, , one-way ANOVA, Tukey^’s test, /group. (d) Brain sample with schematic illustration showing one area in the perihematomal region and the small white square within the coronal section of the brain indicates the location of where the immunofluorescence staining images were taken. (e, f) Representative microphotographs of coimmunofluorescence staining of albumin (green) with neurons (NeuN, red), as well as HO-1 (green) with neurons (NeuN, red) in the ipsilateral perihematomal region at 72 h after ICH (/group), nuclei were stained with DAPI (blue), scale bar = 50 μm, HO-1: heme oxygenase 1; DAPI: 4,6-diamidino-2-phenylindole.

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