Research Article
Effect and Mechanism of LRP6 on Cardiac Myocyte Ferroptosis in Myocardial Infarction
Figure 2
LRP6 deletion promotes ferroptosis in cardiomyocytes. (a) The protein expression of LRP6 in the infarcted myocardial tissue was detected using western blot analysis with SDS-PAGE. Top: typical blots; bottom: pooled data, biological replication indicated five mice in each group. Data were (b) Analysis of expression of LRP6 transfected with LRP6-siR-mus-4649, LRP6-siR-mus-2488, or LRP6-siR-mus-513. Pooled data: biological replication indicated five mice in each group. Data were and compared with ctrl by one-way ANOVA with Bonferroni’s post hoc test. (c) Evaluation of the activity of cardiomyocytes interfered with LRP6 following induction of hypoxia and erastin. Pooled data: biological replication indicated five mice in each group. Data were and compared with ctrl by two-tailed unpaired Student’s -test. (d, e) Representative of the levels of MDA and Fe2+ in cardiomyocytes following induction of hypoxia and erastin in 48 hours. 0 and 10 indicated the concentrations of ferroptosis inducer erastin (μM), respectively. Pooled data: biological replication indicated five mice in each group. Data were and compared with ctrl by one-way ANOVA with Bonferroni’s post hoc test.
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