Bortezomib Rescues Ovariectomy-Induced Bone Loss via SMURF-Mediated Ubiquitination Pathway

Figure 6

The involvement of SMURF1/2 in the bortezomib-mediated antiosteoporotic effect. (a, b) The mRNA levels of smurf1 and smurf2 genes were quantified with real-time RT-PCR using GAPDH as the normalization. (c) The protein levels of SMURF enzymes and SMAD pathway were determined using Western blot assays. (d) Representative immunohistology images stained by SMURF1, SMURF2, and p-SMAD1/5/9. (e–g) Quantification of the cell number of SMURF1-, SMURF2-, or P-SMAD-1/5/9-positive cells. Values represent of six samples in each group () in histological staining assays. Statistically significant differences are indicated by where or where between the indicated groups.

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