Research Article

The Extracts of Angelica sinensis and Cinnamomum cassia from Oriental Medicinal Foods Regulate Inflammatory and Autophagic Pathways against Neural Injury after Ischemic Stroke

Figure 5

TLR4 and nuclear P65 expressions and inflammatory cytokines concentrations in LPS-stimulated BV2 microglial cells. (a) Schematic diagram of LPS-stimulated BV2 microglial cells incubated with CSF and transfected with TLR4 siRNA, as well as TLR4 expression by western blotting. (b) Immunofluorescence staining with nuclear p65 (green) was presented in LPS-induced BV2 microglial cells (magnification, ×400) and positive signal analysis. (c) ELISA assay of TNF-α, IL-1β, and IL-6 in conditional culture medium. Data presented as . vs. LPS; ### vs. LPS + negative TLR4 siRNA. Scale bar 50 μm.
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