Research Article

Hepcidin Promoted Ferroptosis through Iron Metabolism which Is Associated with DMT1 Signaling Activation in Early Brain Injury following Subarachnoid Hemorrhage

Figure 6

Expression of hepcidin, DMT1, FPN1 and GPX4 after different doses of heparin were introduced following SAH. Representative WBs of hepcidin, DMT1, FPN1 and GPX4 (a), densitometric quantification of the optical densities of these protein bands (b-e), all protein expression levels were significantly changed at the dose of 5 mg/kg heparin after SAH, P <0.05 vs the NS + SAH group (, each group). Perl’s iron staining after 5 mg/kg heparin was administered. Positive areas 5 mg/kg heparin decreased, compared with the NS + SAH groups, final magnification 400× (f). The activity of components of ferroptosis (iron content, MDA, GSH, and GPX4) were determined after 5 mg/kg heparin was introduced. Treatment with 5 mg/kg heparin decreased iron content and MDA, while increasing GSH and GPX4 activity and protecting against ferroptosis, compared with the NS + SAH groups (g-j), P <0.05 vs the NS + SAH group (, each group). Expression of hepcidin, DMT1, FPN1, and GPX4 after different doses of OSM were introduced following SAH. Representative WBs of hepcidin, DMT1, FPN1, and GPX4 (k), densitometric quantification of the optical densities of these protein bands (l-o), and all protein expression levels were significantly changed at the dose of 10 μg OSM after SAH, P <0.05 vs. the NS+SAH group (, each group). Perl’s iron staining after 10 μg OSM was administered. Positive areas 10 μg OSM increased, compared with the PBS+SAH groups, final magnification ×400 (p). The activity of components of ferroptosis (iron content, MDA, GSH, and GPX4) were determined after 10 μg OSM was introduced. Treatment with 10 μg OSM increased iron content and MDA, while decreasing GSH and GPX4 activity and aggravating ferroptosis, compared with the PBS+SAH groups (q-t), P <0.05 vs. the PBS+SAH group (, each group).
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