Hepcidin Promoted Ferroptosis through Iron Metabolism which Is Associated with DMT1 Signaling Activation in Early Brain Injury following Subarachnoid Hemorrhage

Table 1

SAH grade and mortality are prognostic indicators, which recorded after surgery and treatment. A total of 30 rats died during or after model constructed due to severe SAH, and another 21 rats were excluded because of their low SAH grade. No death was observed in the sham group, after surgery, with mortality rates (Table 1), which were calculated as follows: sham = 0% (0 of 40), SAH = 27.27% (18 of 66), NS = 25.0% (6 of 24), Hep = 16.67% (6 of 36), Abs = 17.39% (4 of 23), Ebs = 17.65% (9 of 51), PBS = 26.92% (7 of 26), OSM = 21.28% (10 of 47) and PBS + Abs = 25% (4 of 16), OSM + Ebs = 29.17% (7 of 24). However, there were no statistically significant differences in mortality rates between treatment groups.

Groups	Mortality	Excluded	Groups	Mortality	Excluded
Experiment 1			Experiment 2
Sham	0%(0/8)	0	Sham	0% (0/8)	0
SAH (6 h 12 h, 24 h, 48 h, 72 h)	21.8% (7/32)	3	SAH	37.5% (3/8)	1
Experiment 3			SAH + Abs	20% (2/10)	0
Sham	0% (0/8)	0	SAH + Ebs (1 mg/kg)	16.67% (2/12)	1
SAH	37.5%(3/8)	1	SAH + Ebs (2 mg/kg)	23.07 (3/13)	1
SAH + NS	20% (2/10)	0	SAH + Ebs (4 mg/kg)	20% (3/15)	0
SAH + Hep (1 mg/kg)	12.5% (1/8)	1	Experiment 5
SAH + Hep (2.5 mg/kg)	22.22 (2/9)	1	Sham	—	0
SAH + Hep (5 mg/kg)	11.1% (1/9)	0	SAH	—	1
Experiment 4			SAH + PBS + Abs	25% (4/16)	1
Sham	0% (0/8)	0	SAH + OSM (10 μg) + Ebs (4 mg/kg)	29.1% (7/24)
SAH	25% (2/8)	2	Experiment 6		0
SAH + PBS	27.78% (5/18)	0	Sham	0% (0/8)	0
SAH + OSM (100 ng)	33.33% (3/9)	0	SAH	30% (3/10)	1
SAH + OSM(1 μg)	20% (2/10)	1	SAH + NS	28.6% (4/14)	0
SAH + OSM (5 μg)	20% (2/10)	1	SAH + Hep (5 mg/kg)	20% (2/10)	0
SAH + OSM(10 μg)	10% (1/10)	1	SAH + Abs	15.38% (2/13)	1
			SAH + Ebs (4 mg/kg)	9.1% (1/11)	0
			SAH + PBS	20% (2/8)	2
			SAH + OSM (10 μg)	25% (2/8)	1
Total		11	Total		10