Gasotransmitter CO Attenuates Bleomycin-Induced Fibroblast Senescence via Induction of Stress Granule Formation

Figure 2

CORM-A1 attenuates BLM-induced cellular senescence in mouse embryonic fibroblasts. (a) Mouse embryonic fibroblasts (MEFs) were treated with CORM-A1 in a dose-dependent manner (0, 10, 20, 40, and 80 μM) for 6 h, and MTT assay was performed to assess the cell viability. MEFs were pretreated with CORM-A1 (40 μM) for 6 h prior to the stimulation of BLM (25 μg/ml) for 48 h. Then, SA-β-gal staining (b) and γ-H2AX foci (c) were detected, and the mRNA expressions of p21 (d), IL-6 (e), TNF-α (f), and IL-1β (g) were detected by qRT-PCR. Quantitative data are expressed as the ( determined in three independent experiments). , , and . ns: not significant.

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