DNA-Aptamer Raised against Receptor for Advanced Glycation End Products Improves Survival Rate in Septic Mice

Figure 3

Effects of RAGE-aptamer on kidney and liver damage in septic mice. (a, c, e, g, and i) Each left panel shows the representative immunostainings of TNF-α (a), IL-1β (c), IL-6 (e), HMGB1 (g), and KIM-1 (i). Each right panel shows the quantitative data. (b, d, f, h, and j) mRNA levels of TNF-α (b), IL-1β (d), IL-6 (f), HMGB1 (h), and KIM-1 (j) in the kidneys. (k) Urinary KIM-1 levels. (l) Left panels show the representative immunostainings of 8-OHdG. Right panel shows the quantitative data. (m) Urinary 8-OHdG levels. (n) NADPH oxidase-derived superoxide generation. (o–r) mRNA levels of p22phox (o), Nox2 (p), p47phox (q), and p67phox (r) in the kidneys. (s) Left panels show the representative HE staining of kidneys. Right panel shows the scores for tubular degeneration/necrosis. (t) Left panels show the representative HE staining of livers. Right panel shows the quantitative data of necrosis area. per group. ^# and ^## compared with control, respectively. and compared with LPS plus control-aptamer-treated mice, respectively. ^†† compared with LPS+vehicle-treated mice.

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