Ghrelin Alleviates Endoplasmic Reticulum Stress in MC3T3E1 Cells by Inhibiting AMPK Phosphorylation

Figure 3

Effects of different concentrations of ghrelin on ROS production induced by TM. Determination of the fluorescence intensity of intracellular ROS induced by TM which was pretreated with different concentrations of ghrelin by a DCFH-DA fluorescence probe (flow cytometry). (a)–(e) represent the reactive oxygen flow diagram of the control, TM, , , and groups. (f) Histogram of the average fluorescence intensity of ROS in each group. Ghrelin pretreatment for 4 hours, followed by TM (1.5 μg/mL) treatment for 20 hours. Data represents the obtained from three separate experiments. indicates comparison with the control group, indicates , ^# indicates comparison with the TM group, ^## indicates , ^### indicates .

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