BMSC-Derived Exosomes Ameliorate LPS-Induced Acute Lung Injury by miR-384-5p-Controlled Alveolar Macrophage Autophagy

Figure 2

Characterization of BMSC-derived exosomes. (a) Nanoparticle trafficking analysis (NTA) showed the diameters and concentration of exosomes. (b) Transmission electron microscope- (TEM-) analyzed exosomes. The image showed a spheroid shape of approximately 100 nm in diameter. The scale bar represents 100 nm. (c) Western blot-characterized exosomes. BMSC-derived exosome preparation was separated by SDS-polyacrylamide gel electrophoresis, electroblotted to the polyvinylidene fluoride (PVDF) membrane, and probed with exosome markers Alix, CD63, and CD9. (d) Label and track BMSC-derived exosomes. After incubating the labeled Exo-red exosomes with alveolar macrophages for 2 h, the exosome pellet shows strong red fluorescence in the cytoplasm of alveolar macrophages. After incubating the labeled Exo-green exosomes with alveolar macrophages for 24 h, the exosome pellet shows intense green fluorescence in the alveolar macrophages (×400 magnification).

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