BMSC-Derived Exosomes Ameliorate LPS-Induced Acute Lung Injury by miR-384-5p-Controlled Alveolar Macrophage Autophagy

Figure 9

Beclin-1 was one of the target genes of miR-384-5p. (a) The relative levels of miR-384-5p were detected by RT-qPCR after treatment with miR-384-5p mimic, miR-384-5p inhibitor, or the corresponding negative control (NC). (b) The relative mRNA levels of Beclin-1 were detected by RT-qPCR after treatment with miR-384-5p mimic, miR-384-5p inhibitor, or the corresponding NC. (c) The protein levels of Beclin-1 were detected by western blot after treatment with miR-384-5p mimic, miR-384-5p inhibitor, or the corresponding NC. β-Actin levels were measured to ensure equal protein loading. (d) Relative quantification (RQ) analyses of the induction in the protein levels of Beclin-1 showed that the miR-384-5p mimic decreased the protein expression level of Beclin-1, whereas the miR-384-5p inhibitor increased the protein level of the protein. (e) Bioinformatics analyses showing that miR-384-5p targets 3-UTR of Beclin-1 mRNA at one binding site. (f) The relative luciferase value of the interaction between miR-384-5p and the 3-UTR of Beclin-1. HEK 293 cells were transfected with wild-type or mutant 3-UTR luciferase constructs and with miR-384-5p mimic, inhibitor, or the corresponding negative control (NC), as indicated (). Values obtained from three independent experiments are expressed as . vs. the untreated group; vs. the untreated group.

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