PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway

Zhang, Yanzhuo; Lv, Zhe; Liu, Yudong; Cao, Huan; Yang, Jianwang; Wang, Baoshan

doi:https://doi.org/10.1155/2021/9980444

Oxidative Medicine and Cellular Longevity

On this page

Abstract Introduction Materials and Methods Results Discussion Data Availability Conflicts of Interest Supplementary Materials References Copyright Related Articles

Special Issue

Oxidative Stress, Senescence, and Nutrition in Age-Related Diseases

View this Special Issue

Research Article | Open Access

Volume 2021 | Article ID 9980444 | https://doi.org/10.1155/2021/9980444

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway

Yanzhuo Zhang,¹Zhe Lv,¹Yudong Liu,^1,2Huan Cao,¹Jianwang Yang,¹and Baoshan Wang¹

Academic Editor: Cristina Cosentino

Received28 Mar 2021

Accepted14 May 2021

Published02 Jun 2021

Abstract

A growing amount of evidence has confirmed the crucial role of the prolyl isomerase PIN1 in aging and age-related diseases. However, the mechanism of PIN1 in age-related hearing loss (ARHL) remains unclear. Pathologically, ARHL is primarily due to the loss and dysfunction of hair cells (HCs) and spiral ganglion cells (SGCs) in the cochlea. Therefore, in this study, we aimed to investigate the role of PIN1 in protecting hair cells and auditory HEI-OC1 cells from senescence. Enzyme-linked immunosorbent assays, immunohistochemistry, and immunofluorescence were used to detect the PIN1 protein level in the serum of ARHL patients and C57BL/6 mice in different groups, and in the SGCs and HCs of young and aged C57BL/6 mice. In addition, a model of HEI-OC1 cell senescence induced by H₂O₂ was used. Adult C57BL/6 mice were treated with juglone, or juglone and NAC, for 4 weeks. Interestingly, we found that the PIN1 protein expression decreased in the serum of patients with ARHL, in senescent HEI-OC1 cells, and in the cochlea of aged mice. Moreover, under H₂O₂ and juglone treatment, a large amount of ROS was produced, and phosphorylation of p53 was induced. Importantly, PIN1 expression was significantly increased by treatment with the p53 inhibitor pifithrin-α. Overexpression of PIN1 reversed the increased level of p-p53 and rescued HEI-OC1 cells from senescence. Furthermore, PIN1 mediated cellular senescence by the PI3K/Akt/mTOR signaling pathway. In vivo data from C57BL/6 mice showed that treatment with juglone led to hearing loss. Taken together, these findings demonstrated that PIN1 may act as a vital modulator in hair cell and HEI-OC1 cell senescence.

1. Introduction

Age-related hearing loss (ARHL) or presbycusis is a prevalent disease in aging people [1, 2]. Hearing loss occurs in most people as they age. Approximately one-third of people over 65 years old are affected by hearing loss according to the World Health Organization. With the aging of the population worldwide, more than 500 million people suffer ARHL [3]. This condition significantly affects the daily communication of older people, and has been shown to be associated with predisposing cognitive impairment and dementia. ARHL is characterized by an age-dependent decline in auditory function. Pathologically, this condition is primarily due to the loss of hair cells and spiral ganglion cells (SGCs) in the cochlea [4]. However, the exact mechanisms of ARHL remain largely unknown.

Peptidyl-prolyl cis/trans isomerase (PIN1) is a novel postphosphorylation signaling regulator. PIN1 regulates proteins by controlling the structure of phosphoproteins by mediating the isomerization of specific phosphorylated Ser/Thr-Pro motifs. As a molecular timer, PIN1 not only controls cell cycle progression and cell division but also regulates cellular senescence [5–8]. Loss of PIN1 in cultured cells induced senescence [8]. PIN1 knock-out mice showed a senescent phenotype [9]. PIN1 expression declined in the myocardium with aging [10]. All these results indicate that PIN1 is an important antiaging molecule. However, the role of this critical regulatory molecule in hair cells has not been previously examined.

Many previous studies have shown that the PI3K/Akt/mTOR pathway is an important pathway in regulating autophagy and apoptosis in the inner ear cochlea [11–14]. Liu et al. [11] demonstrated that the antioxidant enzyme PRDX1 triggers autophagy in spiral ganglion neurons through activation of the PTEN-Akt signaling pathway. Rapamycin alleviated cisplatin-induced ototoxicity by promoting autophagy [11, 15]. In Fus1 KO mice, researchers found dysregulation of Akt and activation of mTOR in cochleae [16]. Although the PI3K/Akt/mTOR pathways are associated with sensory hair cell survival, the mechanisms through which PI3K/Akt/mTOR regulate hair cell senescence are not fully defined.

HEI-OC1 cells are a hair-cell-like cell line that maintain hair cell characteristics and have been extensively used in many previous studies to investigate the protective mechanism of hair cells [17–21]. Therefore, in this study, we used aging C57BL/6 mice and H₂O₂-treated HEI-OC1 cells to determine the role and mechanism of PIN1 in the senescence of hair cells and HEI-OC1 cells.

2. Materials and Methods

2.1. Participants

The participants were selected from the Second Hospital of Hebei Medical University. Participants aged 30 to 80 years old without hearing loss were included in the control group (27 people aged 30-60 years old; 25 people aged 65-80 years old). In the study, 20 patients who were clinically determined to have age-related SNHL were included as the study group. The exclusion criteria were severe diseases, such as cancer, dementia, and psychiatric disorders.

2.2. Pure Tone Audiometry

Procedures were conducted in a soundproof booth. Sounds were delivered via earphone (TDH-50P). Six frequencies (250 Hz to 8000 Hz) were tested in routine pure tone audiometric examination (OB922 Audiometer, Madsen, Ltd., Denmark). The mean threshold of each frequency was calculated in both ears for each subject individually. The mean individual subject thresholds of 250, 500, and 1000 Hz were averaged to obtain the average pure tone hearing level of low frequencies (PTA) and that of 2000, 4000, and 8000 Hz as the average pure tone hearing level of high frequencies (PTA). HL was defined as .

2.3. Animals

C57BL/6 mice were purchased from the Laboratory Animal Center, Charles River (Beijing China). Mice were divided into two groups: the young group (2 months old) and the old group (12 months old). Two-month-old mice were randomly divided into the control group, the juglone group, the juglone + NAC group, and the DMSO group. There were 6 mice per group. All experiments were performed according to protocols approved by the Animal Research Center, Hebei Medical University.

2.4. Enzyme-Linked Immunosorbent Assay (ELISA)

After centrifugation, serum samples were immediately frozen at -80°C for further analysis. Human PIN1 ELISA kits (Cat# ZC-32747), human ROS ELISA kits (Cat# ZC-33336), mouse PIN1 ELISA kits (Cat# ZC-54798), and mouse ROS ELISA kits (Cat# ZC-38260) were from Shanghai ZCIBIO. Then, the concentrations were measured according to the manufacturer’s instructions. The absorbance at 450 nm was measured using a Synergy2 Automated Enzyme-Linked Immunosorbent Assay (Thermo Fisher Scientific, Inc., USA).

2.5. Cell Culture and Cell Transfection

HEI-OC1 cells (the House Ear Institute-Organ of Corti 1 cell line) were grown under permissive conditions (33°C, 10% CO₂) in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Gibco USA) containing 10% fetal bovine serum (FBS; Gibco BRL) without antibiotics.

HEI-OC1 cells were cultured in 6-well plates and transfected when the cell fusion degree was 70-90%. The Lipofectamine 3000 Reagent was diluted with serum-free DMEM. A master mix of DNA was prepared by diluting DNA with serum-free DMEM medium, then adding the P3000 Reagent, and mixing. The diluted DNA and Lipofectamine 3000 Reagent were mixed (1 : 1) and incubated for 15 minutes at room temperature. DNA-lipid complexes were added to the cell supernatant and incubated for 2 days at 33°C.

2.6. Auditory Brainstem Response

All mice were anesthetized with an intraperitoneal injection (a mixture of ketamine-xylazine: 100 mg/kg ketamine and 10 mg/kg xylazine). Then, platinum needle electrodes were inserted at the vertex (reference electrode), behind the right ear (active electrode), and at the back (ground electrode) of the mice. Auditory brainstem responses (ABR) were measured in response to tone pips of 8, 12, 16, 20, 24, 28, and 32 kHz. ABR recordings were performed with a Tucker Davis Technologies (TDT) System III workstation running in a BioSigRP Soundbooth (IAC Acoustics). The hearing threshold was defined as the lowest intensity to generate a reproducible ABR waveform.

2.7. Immunohistochemistry

C57BL/6 mice were decapitated after the ABR tests. The temporal bones were dissected, and the cochleae were obtained and fixed with 4% paraformaldehyde at 4°C overnight and decalcified in 4% sodium ethylenediaminetetraacetic acid for 3 days at 4°C.

The cochleae were then dehydrated, processed, and embedded in paraffin. Paraffin-embedded specimens were cut to a thickness of 4 μm. We selected morphologically intact slices to conduct immunohistochemistry staining. The selected 4 μm sections were deparaffinized and rehydrated. Antigen recovery was performed by a microwave. Next, the sections were incubated with a primary antibody against PIN1 (10495-1-AP, Proteintech, USA) overnight at 4°C. The following day, after sequential incubation with a biotinylated secondary antibody and horseradish peroxidase conjugated streptavidin for 30 min at 37°C, the sections were stained with 3,3-diaminobenzidine (DAB). The slides were finally dehydrated, cleared, and mounted with coverslips. The negative controls were prepared by replacing the primary antibody with phosphate-buffered saline.

2.8. Immunofluorescence

After decalcification with 4% sodium EDTA solution for 3 days at 4°C, the cochleae were microdissected into three turns (apex, middle, and base). HEI-OC1 cells were fixed with 4% paraformaldehyde. The tissue and cell specimens were first permeabilized in Triton X-100 solution and then blocked with 10% normal goat serum for 30 min at room temperature. After incubation with primary anti-PIN1 (10495-1-AP, Proteintech, USA) antibody overnight at 4°C, the samples were then washed three times with PBS and incubated with secondary fluorescent antibody for 1 h at 37°C in the dark to detect the primary antibody, followed by incubation with Alexa Fluor 488-phalloidin for 1 h at room temperature in the dark.

2.9. Reagent Treatment

HEI-OC1 cells were seeded in six-well plates for each experiment for 24 h. Before H₂O₂ stimulation, the cells were pretreated with the PI3K inhibitor LY294002 (25 μM, HY-10108/CS-0150, MCE, USA), the Akt activator SC79 (4 μg/mL, B5663, APExBIO, USA) and the ROS scavenger NAC (2 mM, G1902071, Aladdin, China) for 1 h; the p53 inhibitor pifithrin-α (10 μM, A4206, APExBIO, USA) was used for 24 h. The PIN1 inhibitor juglone (1, 5, and 10 μM, STBH9858, Sigma-Aldrich, USA) was used for 45 min.

2.10. Senescence-Associated β-Galactosidase Stain

Cellular senescence-associated β-galactosidase (SA-β-Gal) staining was conducted using a senescence β-galactosidase staining kit (Beyotime Institute of Biotechnology, Shanghai, China) following the manufacturer’s instructions. Before staining, cells were gently washed with PBS and fixed with fixing solution. After washing three times with PBS, 1 mL of staining solution was added to each well, sealed with parafilm, and incubated at 37°C without CO₂ overnight.

2.11. Protein Extraction and Western Blotting

The cells were collected and lysed on ice with RIPA buffer and PMSF for 30 min. Detection of phosphorylated proteins requires the addition of phosphorylase inhibitors (04906845001, Roche Switzerland). The supernatant was centrifuged at 12,000 rpm for 20 min at 4°C. The BCA Protein Assay (PC0020, Solarbio, China) was used to determine the protein concentration. The protein extracts from the cells were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred to polyvinylidene fluoride membranes. The membranes were blocked with 5% nonfat milk for 2 h at 37°C and then incubated overnight at 4°C with anti-PIN1 (dilution, 1 : 1000; 10495-1-AP, Proteintech, USA), p53 (dilution, 1 : 1000; 21891-1-AP, Proteintech, USA), p-p53 (dilution, 1 : 1000; 9284, Cell Signaling Technology, USA), p21 (1 : 1000, dilution, 1 : 1000; 27296-1-AP, Proteintech, USA), p-16 (1 : 1000, 10883-1-AP, Proteintech, USA), Akt (1 : 1000, ab8805, Abcam, USA), p-Akt (1 : 1000, ab81283, Abcam, USA), mTOR (1 : 1000, 2983, Cell Signaling Technology, USA), p-mTOR (1 : 1000, 5536, Cell Signaling Technology, USA), and GAPDH (1 : 2000 Proteintech, USA) antibodies. ImageJ software was used to quantify the band intensity. The intensity value of each target band was normalized to that of GAPDH.

2.12. Statistical Analysis

All experiments were independently repeated at least three times. Data are presented as the and were analyzed with SPSS. Student’s -test and one-way ANOVA were used for statistical analysis. Values with were considered significant.

3. Results

3.1. PIN1 and ROS Concentrations in Human and C57BL/6 Mouse Serum

The serum concentrations of PIN1 and ROS were measured in all participants and C57BL/6 mice. ELISAs revealed that ROS expression levels were increased in the patients in the ARHL group and the old C57BL/6 mice (Figures 1(a) and 1(c)). PIN1 expression levels were decreased in the patients and in the old C57BL/6 mice with ARHL (Figures 1(b) and 1(d)).

(a)

(b)

(c)

(d)

3.2. Elevated Auditory Brainstem Response Threshold and Cell Senescence in the Cochleae of the Aged C57BL/6 Mice

We examined the auditory function in young and old mice by measuring ABR. As shown in Figure 2(a), not only was the ABR threshold at a high frequency of the old mice significantly higher than that of the young mice but also that at middle and low frequencies was significantly higher in the old mice than the young mice, which indicated a decline in auditory function in the old mice. In addition, the results showed that senescence-associated β-galactosidase- (SA-β-gal-) positive cells in the aged mice were more abundant than those in the young mice in spiral ganglion cells (SGCs) (Figure 2(b)) and hair cells (HCs) (Figure 2(c)).

(a)

(b)

(c)

3.3. The Expression of PIN1 Is Downregulated in the Cochleae of the Aged C57BL/6 Mice

We examined PIN1 expression in the cochlea of the young and old mice. IF and IHC analysis showed that the PIN1 protein was expressed in the cytoplasm and nucleus of HCs (Figures 3(a) and 3(b)) and SGCs (Figure 3(c)). PIN1 expression was markedly decreased in the old mice compared to the young mice (Figures 3(a)–3(e)). Linear analysis showed that the expression of PIN1 in the cochlea was negatively correlated with hearing threshold (Figures 3(f)–3(h)). Taken together, the results showed that PIN1 might be involved in ARHL.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

3.4. Downregulation of PIN1 Expression in HEI-OC1 Cells Induced by H₂O₂

The different concentrations of H₂O₂ on cell viability were detected by the MTS method. After 2 h of stimulation by H₂O₂, the relative cell survival rate was observed and was calculated (Supplementary Figure 1). Therefore, HEI-OC1 cells were exposed to H₂O₂ (1 mM for 2 h) to induce cellular senescence. Thereafter, senescence-associated SA-β-Gal staining was applied to detect senescent HEI-OC1 cells. As shown in Figure 4(a), the percentage of cells positive for SA-β-Gal was increased in the HEI-OC1 cells treated with H₂O₂. Additionally, H₂O₂ significantly elevated the expression of p-p53, p21, and p16 compared with that in the control group (Figure 4(b)). However, PIN1 protein expression, detected by Western blotting (Figure 4(c)) or immunofluorescence (Figure 4(d)), was significantly decreased in the HEI-OC1 cells treated with H₂O₂.

(a)

(b)

(c)

(d)

3.5. PIN1 Modulates HEI-OC1 Cell Senescence

To better understand the role of PIN1 in the cellular senescence of HEI-OC1 cells, we transfected the HA-PIN1 overexpression plasmid into HEI-OC1 cells or treated HEI-OC1 cells with juglone, a PIN1 inhibitor [22]. The transfection effect is shown in Figures 5(a) and 5(c). As illustrated in Figures 5(e) and 5(g), PIN1 overexpression caused a significant reduction in the expression of p-p53, p21, and p16. The percentage of SA-β-gal-positive cells was also reduced in the PIN1 overexpression group (Figures 5(i) and 5(j)). In contrast, juglone inhibited PIN1 in a concentration-dependent manner (Figures 5(b) and 5(g)) and accelerated senescence in HEI-OC1 cells in a concentration-dependent manner (Figures 5(f), 5(h), 5(i), and 5(j)). However, these changes were not found in the different doses of solvent control group (Supplementary Figures 2(A) and 2(B)).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

3.6. PIN1 Mediates HEI-OC1 Cell Senescence by Affecting the PI3K/Akt/mTOR Signaling Pathway

Western blotting results showed that PIN1 overexpression rescued the H₂O₂-induced upregulation of p-Akt and p-mTOR expression in HEI-OC1 cells (Figure 6(a)). However, PIN1 inhibition induced upregulation of p-Akt and p-mTOR expression (Figures 7(a) and 7(b)), and the expression of p-Akt and p-mTOR did not change in the different doses of the solvent control group (Supplementary Figures 2(C) and 2(D)). Then, to further study the role of the PI3K/Akt/mTOR signaling pathway in HEI-OC1 cell senescence, we pretreated HEI-OC1 cells with SC79 (Akt activator) or LY294002 (Akt inhibitor) before H₂O₂ stimulation. The results showed that neither SC79 nor LY294002 changed the PIN1 protein levels (Figures 6(b) and 6(d)). SC79 upregulated the expression of p-p53, p16, and p21 (Figure 6(c)) and increased the number of SA-β-gal-positive cells (Figure 6(f)). However, LY294002 had an opposite effect compared with SC79 (Figures 6(e) and 6(f)). These results indicated that the protective role of PIN1 could be mediated by inhibiting the PI3K/Akt/mTOR pathway in senescent HEI-OC1 cells.

(a)

(b)

(c)

(d)

(e)

(f)

Figure 6

PIN1 mediates HEI-OC1 cell senescence by affecting the PI3K/Akt/mTOR signaling pathway. (a) Western blots for the expression of p-Akt, Akt, p-mTOR, and mTOR in HEI-OC1 cells after transfection with the HA-PIN1 plasmid. (b) Western blots for the expression of p-Akt, Akt, p-mTOR, mTOR, and PIN1 in HEI-OC1 cells after pretreatment with SC79 (Akt activator). (c) Western blots for the expression of p53, p-p53, p21, and p16 after pretreatment with SC79. (d) Western blots for the expression of p-Akt, Akt, p-mTOR, mTOR, and PIN1 in HEI-OC1 cells after pretreatment with LY294002 (an inhibitor of PI3K/Akt). (e) Western blots for the expression of p53, p-p53, p21, and p16 after pretreatment with LY294002. (f) SA-β-gal staining for HEI-OC1 cells ( vs. control group, ^# vs. DMSO+H₂O₂ group, ^& vs. H₂O₂ group. ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

3.7. H₂O₂ Affects the Expression of PIN1 through p53, which In Turn Affects Senescence

To further investigate the regulatory mechanism of PIN1 in ARHL, we used the p53 inhibitor pifithrin-α (PFT-α) to evaluate whether H₂O₂ affects the expression of PIN1 through p53. When HEI-OC1 cells were incubated with PFT-α, the p-p53 protein level was decreased, but the PIN1 protein level significantly increased (Figures 7(c) and 7(d)). In addition, we investigated the expression of senescence-associated proteins. The results showed that pretreatment of the cells with PFT-α caused a significant decrease in the expression of p21 and p16 (Figures 7(c) and 7(e)), and the percentage of SA-β-gal-positive cells was also reduced (Figures 7(f) and 7(g)). The results suggested that H₂O₂ inhibited the expression of PIN1 by upregulating p53 expression, which in turn affected senescence.

3.8. H₂O₂ Affects the Expression and Activity of p53 through ROS and Then Affects Senescence

In the model of senescence and in the juglone treatment group, high levels of ROS were produced (Figures 8(a) and 8(b)). To detect whether ROS affect the expression and activity of p53, we used the ROS inhibitor N-acetyl-L-cysteine (NAC). The results showed that the production of ROS almost recovered to the level of the control group after NAC treatment (Figures 8(a) and 8(b)). NAC led to a restoration of p-p53 levels (Figures 8(c)–8(f)). In addition, when cells were pretreated with NAC, the expression level of PIN1 was increased (Figures 8(c) and 8(e)), and the expression levels of p21 and p16 were decreased (Figures 8(c)–8(f)). The percentage of SA-β-gal-positive cells was also reduced by NAC treatment (Figures 8(g) and 8(h)).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

Figure 8

H₂O₂ affects the expression and activity of p53 through ROS and then affects senescence. (a, b) Intracellular ROS levels were measured by DCFH-DA in HEI-OC1 cells (ns: nonsignificant; vs. control group, ^# vs. H₂O₂ group). (c–f) Western blots for the expression of p53, p-p53, p21, p16, and PIN1 after pretreatment with the ROS inhibitor N-acetyl-L-cysteine (NAC) ( vs. the DMSO group, ^# vs. H₂O₂ group, ^& vs. the juglone group). (g, h) SA-β-gal staining for HEI-OC1 cells ( vs. the control group, ^# vs. the H₂O₂ group, ^& vs. the juglone group. ).

3.9. Juglone Treatment Results in C57BL/6 Mouse Hearing Loss

To further study whether the reduction in PIN1 expression could induce hair cell senescence, we treated the mice with juglone, or juglone and NAC at the same time. Intraperitoneal injection of juglone (1 mg/kg, three times a week for 4 weeks) and/or NAC (1.5 g/kg/d, oral, for 4 weeks) was performed. As shown in Figures 9(a) and 9(b), in the juglone-treated group, the ROS level expression increased in the serum compared with the control group and the juglone + NAC group. In the juglone + NAC treatment group, the PIN1 level expression increased in the serum compared with the juglone group. Moreover, in the juglone-treated group, ABR thresholds at all frequencies were increased, especially at high frequency. Compared with the juglone-treated group, the juglone + NAC group had significantly decreased ABR thresholds at high frequencies (Figure 9(c)). In the basal turn hair cells, there were more SA-β-gal-positive cells in the juglone-treated group than in the other groups (Figure 9(d)).

(a)

(b)

(c)

(d)

Figure 9

Juglone treatment induces C57BL/6 mouse hearing loss. (a) ROS level in the C57BL/6 mice serum. (b) PIN1 levels in the C57BL/6 mice serum. (c) Compared with the other groups, the juglone-treated group had significantly increased ABR thresholds at all frequencies, especially at high frequencies. Compared with the juglone-treated group, the juglone + NAC group had significantly decreased ABR thresholds at high frequencies. (d) In the basal turn hair cells, SA-β-gal-positive cells in the juglone-treated group were greater than those in the other groups ( vs. the DMSO group; ^# vs. the juglone group. ).

4. Discussion

Sensorineural hearing loss is caused by many factors that impair hair cell and spiral ganglion neuron function [23–28], including ototoxic drugs, genetic factors, aging, noise exposure, and chronic cochlear infections [29–33]. A growing amount of evidence has demonstrated that PIN1 plays critical roles in aging-associated diseases [34–39]. PIN1-deficient mice displayed signs of premature senility, such as rapid telomere shortening and loss of motor coordination and behavioral defects and neuronal loss [7]. It has been reported that overexpression of PIN1 rescues cellular senescence of atherosclerotic VSMCs and downregulates the expression of p53 and p21 [40]. Toko et al. [10] reported that knockdown of PIN1 led to cellular senescence, but overexpression of PIN1 inhibited senescence of cardiac progenitor cells. However, the role of PIN1 in ARHL has not been previously reported. For this research, PIN1 protein expression was markedly reduced in the serum of patients with ARHL, SGCs, and HCs of old mice and senescent HEI-OC1 cells. The expression of PIN1 in the cochlea was negatively correlated with the hearing threshold. In vitro, the overexpression of PIN1 inhibited the senescence of HEI-OC1 cells. Inhibited PIN1 accelerated senescence in HEI-OC1 cells. In vivo, we found that treatment with juglone led to hearing loss in C57BL/6 mice, especially at high frequencies. All of these results support our hypothesis that PIN1 modulates HEI-OC1 and hair cell senescence.

Astle et al. reported that the activation of the PI3K/Akt signaling pathway induces cell senescence, and mTOR is a related mediator [41]. In this signaling pathway, mTOR is a downstream target of the PI3K/Akt signaling pathway, and Akt is a positive regulator of mTOR [42]. Studies have shown that topical application of the mTOR inhibitor rapamycin can reduce p16 protein expression and cell aging in skin tissue [43]. Hu et al. [44] demonstrated that Akt/mTOR activity was increased upon aging in zebrafish retinas, and increased Akt/mTOR activity is a major player in age-related retinal neuropathy in zebrafish. It was also demonstrated that gentamicin resulted in the activation of Akt in the organ of Corti and in spiral ganglion cells [45]. The mTOR inhibitor rapamycin protected sensory hair cells against gentamicin. Furthermore, rapamycin attenuates noise-induced hair cell loss by reducing oxidative stress [46]. Nevertheless, other studies suggest that PI3K/Akt signaling is a protective mechanism of the inner ear. It was reported that PI3K/Akt is an endogenous protective mechanism of the inner ear. The activation of PI3K/Akt protects mice from cochlear injury and hearing damage induced by gentamicin and noise [47, 48]. Inhibition of mTOR results in auditory hair cell damage and decreased spiral ganglion neuron outgrowth [49]. Inhibition of the PI3K/Akt pathway can induce senescence and directly increase the expression of p21 [50, 51]. In this article, we showed that the expression of p-Akt and p-mTOR in HEI-OC1 cells treated with H₂O₂ and juglone substantially increased. Inhibition of the PI3K/Akt/mTOR pathway significantly alleviated H₂O₂-induced senescence in HEI-OC1 cells. These results suggest that the activation of Akt aggravated senescence in HEI-OC1 cells.

In multiple cancer types, many studies have shown that the expression levels of PIN1 and levels of Akt phosphorylation are strongly correlated. However, experiments have shown that in mouse periodontal tissues, PIN1 siRNA enhanced mTOR and Akt activation, which was attenuated by PIN1 overexpression [52]. In the present study, PIN1 overexpression caused a reduction in the expression of p-Akt. The inhibition of PIN1 caused upregulation of p-Akt and p-mTOR expression. Furthermore, the activation of Akt aggravated H₂O₂-induced senescence in HEI-OC1 cells. All of these results suggested that PIN1 protects HEI-OC1 cells from senescence by inhibiting the PI3K/Akt/mTOR signaling pathway.

p53 plays a crucial role in the process of cellular senescence. p53 initiates various stress responses, including cell cycle arrest and aging [53]. The overexpression of p53 promotes aging. Our data showed that the expression of p-p53 was significantly higher in the H₂O₂-induced HEI-OC1 cells than in the control cells. Jeong et al. [54] reported that endoplasmic reticulum (ER) stress decreased PIN1 expression through p53 activation, and overexpression of p53 observably decreased PIN1 expression in HCT116 cells. However, the regulators of PIN1 expression during aging are still not fully understood, especially in ARHL. In our study, when the cells were treated with the p53 inhibitor pifithrin-α, PIN1 expression was significantly increased, so we hypothesized that H₂O₂ might regulate PIN1 expression through p53. p-p53 expression was significantly decreased by PIN1 overexpression. These findings suggest that PIN1 and p53 regulate each other. However, the exact mechanism remains to be determined.

The gradual accumulation of ROS is a key mediator during the aging process. In the present study, H₂O₂ and juglone treatment resulted in an increase in ROS activity, indicating that H₂O₂ induced senescence via the production of ROS, and inhibition of PIN1 caused increased ROS production also. The ROS scavenger NAC could reduce ROS production, reverse changes in aging-related biomarkers, protect cells from ROS-induced cellular senescence, and indirectly inhibit mTOR [55–57]. In this study, we also found that NAC reduced the ROS production and reversed changes in aging-related biomarkers. In PDAC cells, silencing PIN1 significantly increased intracellular ROS [58]. In this study, we speculate that significantly decreased PIN1 expression may increase ROS production.

Collectively, under H₂O₂ treatment (or PIN1 inhibition), a large amount of ROS was produced, and the generation of ROS upregulated p-p53 expression. Then, p-p53 negatively regulated PIN1 expression, and overexpression of PIN1 reversed the increase in p-p53. More importantly, PIN1 mediated cellular senescence by affecting the PI3K/Akt/mTOR signaling pathway (Figure 10).

Above all, our findings, for the first time, demonstrated that PIN1 expression is downregulated in the serum of patients with ARHL, in the cochleae of aged C57BL/6 mice, and in senescent HEI-OC1 cells. The in vivo data showed that PIN1 inhibition led to hearing loss. However, only animal and cellular experiments were conducted, and clinical data and conclusions are lacking. These limitations need to be addressed in the future.

Data Availability

Data can be found in the manuscript and supplementary information files.

Conflicts of Interest

All authors declare that they have no conflicts of financial interest.

Supplementary Materials

Supplementary 1. Supplementary Figure 1: the different concentrations of H₂O₂ on cell viability were detected by the MTS method. After stimulation by H₂O₂ for 2 h, we observed the relative cell survival rate and calculated .

Supplementary 2. Supplementary Figure 2: (A, B) Western blot for the expression of p53, p-p53, p21, and p16 in HEI-OC1 cells after treatment with DMSO (0.1, 0.5, and 1 μL); (C, D) Western blot for the expression of p-Akt, Akt, p-mTOR, and mTOR in HEI-OC1 cells after treatment with DMSO (0.1, 0.5, and 1 μL).

References

Z. H. He, S. Y. Zou, M. Li et al., “The nuclear transcription factor FoxG1 affects the sensitivity of mimetic aging hair cells to inflammation by regulating autophagy pathways,” Redox Biology, vol. 28, article 101364, 2020.
View at: Publisher Site | Google Scholar
X. Fu, X. Sun, L. Zhang et al., “Tuberous sclerosis complex-mediated mTORC1 overactivation promotes age-related hearing loss,” Journal of Clinical Investigation, vol. 128, no. 11, pp. 4938–4955, 2018.
View at: Publisher Site | Google Scholar
WHO, Addressing the Rising Prevalence of Hearing Loss, World Health Organization, Geneva, Switzerland, 2018.
S. H. Sha, A. Kanicki, G. Dootz et al., “Age-related auditory pathology in the CBA/J mouse,” Hearing Research, vol. 243, no. 1–2, pp. 87–94, 2008.
View at: Publisher Site | Google Scholar
R. La Montagna, I. Caligiuri, A. Giordano, and F. Rizzolio, “Pin1 and nuclear receptors: a new language?” Journal of Cellular Physiology, vol. 228, no. 9, pp. 1799–1801, 2013.
View at: Publisher Site | Google Scholar
H. Toko, N. Hariharan, M. H. Konstandin et al., “Differential Regulation of Cellular Senescence and Differentiation by Prolyl Isomerase Pin1 in Cardiac Progenitor Cells,” The Journal of Biological Chemistry, vol. 289, no. 9, pp. 5348–5356, 2014.
View at: Publisher Site | Google Scholar
T. H. Lee, L. Pastorino, and K. P. Lu, “Peptidyl-prolyl cis-trans isomerase Pin1 in ageing, cancer and Alzheimer disease,” Expert Reviews in Molecular Medicine, vol. 13, article e21, 2011.
View at: Publisher Site | Google Scholar
K. Wheaton, J. Muir, W. Ma, and S. Benchimol, “BTG2 antagonizes Pin1 in response to mitogens and telomere disruption during replicative senescence,” Aging Cell, vol. 9, no. 5, pp. 747–760, 2010.
View at: Publisher Site | Google Scholar
T. H. Lee, A. Tun-Kyi, R. Shi et al., “Essential role of Pin1 in the regulation of TRF1 stability and telomere maintenance,” Nature Cell Biology, vol. 11, no. 1, pp. 97–105, 2009.
View at: Publisher Site | Google Scholar
H. Toko, M. H. Konstandin, S. Doroudgar et al., “Regulation of cardiac hypertrophic signaling by prolyl isomerase Pin1,” Circulation Research, vol. 112, no. 9, pp. 1244–1252, 2013.
View at: Publisher Site | Google Scholar
W. Liu, L. Xu, X. Wang et al., “PRDX1 activates autophagy via the PTEN-AKT signaling pathway to protect against cisplatin-induced spiral ganglion neuron damage,” Autophagy, vol. 12, pp. 1–23, 2021.
View at: Google Scholar
S. Zhang, Y. Zhang, Y. Dong et al., “Knock-down of Foxg1 in supporting cells increases the trans-differentiation of supporting cells into hair cells in the neonatal mouse cochlea,” Cellular and Molecular Life Sciences, vol. 77, no. 7, article 3291, pp. 1401–1419, 2020.
View at: Publisher Site | Google Scholar
H. Zhou, X. Qian, N. Xu et al., “Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice,” Cell Death & Disease, vol. 11, no. 10, article 3110, p. 913, 2020.
View at: Publisher Site | Google Scholar
Z. He, L. Guo, Y. Shu et al., “Autophagy protects auditory hair cells against neomycin-induced damage,” Autophagy, vol. 13, no. 11, pp. 1884–1904, 2017.
View at: Publisher Site | Google Scholar
B. Fang and H. Xiao, “Rapamycin alleviates cisplatin-induced ototoxicity in vivo,” Biochemical and Biophysical Research Communications, vol. 448, no. 4, pp. 443–447, 2014.
View at: Publisher Site | Google Scholar
W. J. T. Tan, L. Song, M. Graham et al., “Novel role of the mitochondrial protein Fus1 in protection from premature hearing loss via regulation of oxidative stress and nutrient and energy sensing pathways in the inner ear,” Antioxidants & Redox Signaling, vol. 27, no. 8, pp. 489–509, 2017.
View at: Publisher Site | Google Scholar
Z. Zhong, X. Fu, H. Li et al., “Citicoline protects auditory hair cells against neomycin-induced damage,” Frontiers in Cell and Developmental Biology, vol. 8, p. 712, 2020.
View at: Publisher Site | Google Scholar
S. Gao, C. Cheng, M. Wang et al., “Blebbistatin inhibits neomycin-induced apoptosis in hair cell-like HEI-OC-1 cells and in cochlear hair cells,” Frontiers in Cellular Neuroscience, vol. 13, p. 590, 2020.
View at: Publisher Site | Google Scholar
Z. H. He, M. Li, Q. J. Fang et al., “FOXG1 promotes aging inner ear hair cell survival through activation of the autophagy pathway,” Autophagy, vol. 19, pp. 1–22, 2021.
View at: Publisher Site | Google Scholar
Y. Zhang, W. Li, Z. He et al., “Pre-treatment with Fasudil prevents neomycin-induced hair cell damage by reducing the accumulation of reactive oxygen species,” Frontiers in Molecular Neuroscience, vol. 12, p. 264, 2019.
View at: Publisher Site | Google Scholar
A. Li, D. You, W. Li et al., “Novel compounds protect auditory hair cells against gentamycin-induced apoptosis by maintaining the expression level of H3K4me2,” Drug Delivery, vol. 25, no. 1, pp. 1033–1043, 2018.
View at: Publisher Site | Google Scholar
A. A. Mesalam, M. el-Sheikh, M. D. Joo et al., “Induction of oxidative stress and mitochondrial dysfunction by juglone affects the development of bovine oocytes,” International Journal of Molecular Sciences, vol. 22, no. 1, pp. 168–214, 2020.
View at: Publisher Site | Google Scholar
W. Liu, X. Xu, Z. Fan et al., “Wnt signaling activates TP53-Induced Glycolysis and Apoptosis Regulator and protects against cisplatin-induced spiral ganglion neuron damage in the mouse cochlea,” Antioxidants & Redox Signaling, vol. 30, no. 11, pp. 1389–1410, 2019.
View at: Publisher Site | Google Scholar
J. Qi, Y. Liu, C. Chu et al., “A cytoskeleton structure revealed by super-resolution fluorescence imaging in inner ear hair cells,” Cell Discovery, vol. 5, no. 1, p. 12, 2019.
View at: Publisher Site | Google Scholar
C. Cheng, Y. Wang, L. Guo et al., “Age-related transcriptome changes in Sox2+ supporting cells in the mouse cochlea,” Stem Cell Research & Therapy, vol. 10, no. 1, article 1437, p. 365, 2019.
View at: Publisher Site | Google Scholar
L. Liu, Y. Chen, J. Qi et al., “Wnt activation protects against neomycin-induced hair cell damage in the mouse cochlea,” Cell Death & Disease, vol. 7, no. 3, article e2136, 2016.
View at: Publisher Site | Google Scholar
R. Guo, M. Xiao, W. Zhao et al., “2D Ti₃C₂T_xMXene couples electrical stimulation to promote proliferation and neural differentiation of neural stem cells,” Acta Biomaterialia, vol. S1742-7061, no. 20, pp. 30749–30752, 2020.
View at: Publisher Site | Google Scholar
R. Guo, S. Zhang, M. Xiao et al., “Accelerating bioelectric functional development of neural stem cells by graphene coupling: implications for neural interfacing with conductive materials,” Biomaterials, vol. 106, pp. 193–204, 2016.
View at: Publisher Site | Google Scholar
F. Tan, C. Chu, J. Qi et al., “AAV-ie enables safe and efficient gene transfer to inner ear cells,” Nature Communications, vol. 10, no. 1, article 11687, p. 3733, 2019.
View at: Publisher Site | Google Scholar
Y. Liu, J. Qi, X. Chen et al., “Critical role of spectrin in hearing development and deafness,” Science Advances, vol. 5, no. 4, article eaav7803, 2019.
View at: Publisher Site | Google Scholar
J. Lv, X. Fu, Y. Li et al., “Deletion of Kcnj16 in mice does not alter auditory function,” Frontiers in Cell and Developmental Biology, vol. 9, article 630361, 2021.
View at: Publisher Site | Google Scholar
F. Qian, X. Wang, Z. Yin et al., “The slc4a2b gene is required for hair cell development in zebrafish,” Aging, vol. 12, no. 19, article 103840, pp. 18804–18821, 2020.
View at: Publisher Site | Google Scholar
Z. He, Q. Fang, H. Li et al., “The role of FOXG1 in the postnatal development and survival of mouse cochlear hair cells,” Neuropharmacology, vol. 144, pp. 43–57, 2019.
View at: Publisher Site | Google Scholar
K. P. Lu and X. Z. Zhou, “The prolyl isomerase PIN1: a pivotal new twist in phosphorylation signalling and disease,” Nature Reviews. Molecular Cell Biology, vol. 8, no. 11, pp. 904–916, 2007.
View at: Publisher Site | Google Scholar
F. Fagiani, S. Govoni, M. Racchi, and C. Lanni, “The peptidyl-prolyl isomerase Pin1 in neuronal signaling: from neurodevelopment to neurodegeneration,” Molecular Neurobiology, vol. 58, no. 3, article 2179, pp. 1062–1073, 2021.
View at: Publisher Site | Google Scholar
Y. C. Liou, A. Ryo, H. K. Huang et al., “Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes,” Proceedings of the National Academy of Sciences, vol. 99, no. 3, pp. 1335–1340, 2002.
View at: Publisher Site | Google Scholar
L. Pastorino, A. Sun, P. J. Lu et al., “The prolyl isomerase Pin1 regulates amyloid precursor protein processing and amyloid-β production,” Nature, vol. 440, no. 7083, pp. 528–534, 2006.
View at: Publisher Site | Google Scholar
J. A. Driver, X. Z. Zhou, and K. P. Lu, “Pin1 dysregulation helps to explain the inverse association between cancer and Alzheimer’s disease,” Biochimica et Biophysica Acta, vol. 1850, no. 10, pp. 2069–2076, 2015.
View at: Publisher Site | Google Scholar
F. W. Atchison, B. Capel, and A. R. Means, “Pin1 regulates the timing of mammalian primordial germ cell proliferation,” Development, vol. 130, no. 15, pp. 3579–3586, 2003.
View at: Publisher Site | Google Scholar
L. Lv, M. Ye, R. Duan et al., “Downregulation of Pin1 in human atherosclerosis and its association with vascular smooth muscle cell senescence,” Journal of Vascular Surgery, vol. 68, no. 3, pp. 873–883.e5, 2018.
View at: Publisher Site | Google Scholar
M. V. Astle, K. M. Hannan, P. Y. Ng et al., “AKT induces senescence in human cells via mTORC1 and p53 in the absence of DNA damage: implications for targeting mTOR during malignancy,” Oncogene, vol. 31, no. 15, pp. 1949–1962, 2012.
View at: Publisher Site | Google Scholar
N. Hay and N. Sonenberg, “Upstream and downstream of mTOR,” Genes & Development, vol. 18, no. 16, pp. 1926–1945, 2004.
View at: Publisher Site | Google Scholar
C. L. Chung, I. Lawrence, M. Hoffman et al., “Topical rapamycin reduces markers of senescence and aging in human skin: an exploratory, prospective, randomized trial,” Geroscience, vol. 41, no. 6, pp. 861–869, 2019.
View at: Publisher Site | Google Scholar
N. Wang, Z. Luo, M. Jin et al., “Exploration of age-related mitochondrial dysfunction and the anti-aging effects of resveratrol in zebrafish retina,” Aging (Albany NY), vol. 11, no. 10, article 101966, pp. 3117–3137, 2019.
View at: Publisher Site | Google Scholar
U. R. Heinrich, S. Strieth, I. Schmidtmann, K. Helling, and H. Li, “Gentamicin alters Akt-expression and its activation in the guinea pig cochlea,” Neuroscience, vol. 311, pp. 490–498, 2015.
View at: Publisher Site | Google Scholar
E. Ebnoether, A. Ramseier, M. Cortada, S. Levano-Huaman, and D. Bodmer, “Sesn2 gene ablation enhances susceptibility to gentamicin-induced hair cell death via modulation of AMPK/mTOR signaling,” Cell Death Discovery, vol. 3, no. 1, article 17024, 2017.
View at: Publisher Site | Google Scholar
J. Chen, H. Yuan, A. E. Talaska, S. H. Sha, and K. Hill, “Increased sensitivity to noise-induced hearing loss by blockade of endogenous PI3K/Akt signaling,” Journal of the Association for Research in Otolaryngology, vol. 16, no. 3, pp. 347–356, 2015.
View at: Publisher Site | Google Scholar
W. Y. Zhu, X. Jin, Y. C. Ma, and Z. B. Liu, “MIF protects against oxygen-glucose deprivation-induced ototoxicity in HEI-OC1 cochlear cells by enhancement of Akt-Nrf2-HO-1 pathway,” Biochemical and Biophysical Research Communications, vol. 503, no. 2, pp. 665–670, 2018.
View at: Publisher Site | Google Scholar
K. Leitmeyer, A. Glutz, V. Radojevic et al., “Inhibition of mTOR by rapamycin results in auditory hair cell damage and decreased spiral ganglion neuron outgrowth and neurite formation in vitro,” BioMed research international, vol. 2015, Article ID 925890, 10 pages, 2015.
View at: Publisher Site | Google Scholar
K. Breitschopf, A. M. Zeiher, and S. Dimmeler, “Pro-atherogenic factors induce telomerase inactivation in endothelial cells through an Akt-dependent mechanism,” FEBS Letters, vol. 493, no. 1, pp. 21–25, 2001.
View at: Publisher Site | Google Scholar
S. Courtois-Cox, S. M. Genther Williams, B. W. Johnson et al., “A negative feedback signaling network underlies oncogene-induced senescence,” Cancer Cell, vol. 10, no. 6, pp. 459–472, 2006.
View at: Publisher Site | Google Scholar
K. H. Park, E. H. Cho, W. J. Bae et al., “Role of PIN1 on in vivo periodontal tissue and in vitro cells,” Journal of Periodontal Research, vol. 52, no. 3, pp. 617–627, 2017.
View at: Publisher Site | Google Scholar
Ö. Demir, E. P. Barros, T. L. Offutt, R. E. Amaro, and M. Rosenfeld, “An integrated view of p53 dynamics, function, and reactivation,” Current Opinion in Structural Biology, vol. 67, pp. 187–194, 2021.
View at: Publisher Site | Google Scholar
K. Jeong, S. J. Kim, Y. Oh et al., “p53 negatively regulates Pin1 expression under ER stress,” Biochemical and Biophysical Research Communications, vol. 454, no. 4, pp. 518–523, 2014.
View at: Publisher Site | Google Scholar
S. Saxena, H. Vekaria, P. G. Sullivan, and A. W. Seifert, “Connective tissue fibroblasts from highly regenerative mammals are refractory to ROS-induced cellular senescence,” Nature Communications, vol. 10, no. 1, article 12398, p. 4400, 2019.
View at: Publisher Site | Google Scholar
M. C. Marazita, A. Dugour, M. D. Marquioni-Ramella, A. M. Suburo, and J. M. Figueroa, “Oxidative stress-induced premature senescence dysregulates VEGF and CFH expression in retinal pigment epithelial cells: implications for age-related macular degeneration,” Redox Biology, vol. 7, pp. 78–87, 2016.
View at: Publisher Site | Google Scholar
D. F. Xue, S. T. Pan, G. Huang, and J. X. Qiu, “ROS enhances the cytotoxicity of cisplatin by inducing apoptosis and autophagy in tongue squamous cell carcinoma cells,” The International Journal of Biochemistry & Cell Biology, vol. 122, article 105732, 2020.
View at: Publisher Site | Google Scholar
C. Liang, S. Shi, M. Liu et al., “PIN1 maintains redox balance via the c-Myc/NRF2 axis to counteract Kras-induced mitochondrial respiratory injury in pancreatic cancer cells,” Cancer research, vol. 79, no. 1, pp. 133–145, 2019.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2021 Yanzhuo Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

1116

Downloads

1130

Citations

Oxidative Medicine and Cellular Longevity

Oxidative Stress, Senescence, and Nutrition in Age-Related Diseases

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway

Abstract

1. Introduction

2. Materials and Methods

2.1. Participants

2.2. Pure Tone Audiometry

2.3. Animals

2.4. Enzyme-Linked Immunosorbent Assay (ELISA)

2.5. Cell Culture and Cell Transfection

2.6. Auditory Brainstem Response

2.7. Immunohistochemistry

2.8. Immunofluorescence

2.9. Reagent Treatment

2.10. Senescence-Associated β-Galactosidase Stain

2.11. Protein Extraction and Western Blotting

2.12. Statistical Analysis

3. Results

3.1. PIN1 and ROS Concentrations in Human and C57BL/6 Mouse Serum

3.2. Elevated Auditory Brainstem Response Threshold and Cell Senescence in the Cochleae of the Aged C57BL/6 Mice

3.3. The Expression of PIN1 Is Downregulated in the Cochleae of the Aged C57BL/6 Mice

3.4. Downregulation of PIN1 Expression in HEI-OC1 Cells Induced by H2O2

3.5. PIN1 Modulates HEI-OC1 Cell Senescence

3.6. PIN1 Mediates HEI-OC1 Cell Senescence by Affecting the PI3K/Akt/mTOR Signaling Pathway

3.7. H2O2 Affects the Expression of PIN1 through p53, which In Turn Affects Senescence

3.8. H2O2 Affects the Expression and Activity of p53 through ROS and Then Affects Senescence

3.9. Juglone Treatment Results in C57BL/6 Mouse Hearing Loss

4. Discussion

Data Availability

Conflicts of Interest

Supplementary Materials

References

Copyright

3.4. Downregulation of PIN1 Expression in HEI-OC1 Cells Induced by H₂O₂

3.7. H₂O₂ Affects the Expression of PIN1 through p53, which In Turn Affects Senescence

3.8. H₂O₂ Affects the Expression and Activity of p53 through ROS and Then Affects Senescence