The Effects of RBP4 and Vitamin D on the Proliferation and Migration of Vascular Smooth Muscle Cells via the JAK2/STAT3 Signaling Pathway

Figure 5

The expressions of JAK2, STAT3, cylinD1 and Bcl-2 were increased in diabetic rats intraperitoneally injected with RBP4, which can be improved after vitamin D supplementation. (a) Immunofluorescence was performed to detect JAK2 (green) in aortic tissue, and blue indicates DAPI-stained nuclei (scale bar, 25 μm). The corresponding OD values are presented ( for group DM and group DAS compared to group NC, ^# for groups VDAS compared to group DAS). (b) Immunofluorescence was performed to detect STAT3 (green) in aortic tissue, and blue indicates DAPI-stained nuclei (scale bar, 25 μm). The corresponding OD values are presented ( for group DM and group DAS compared to group NC, ^# for groups VDAS compared to group DAS). (c) The expressions of JAK2, STAT3, cyclinD1, and Bcl-2 were analyzed by qRT-PCR ( for group DM and group DAS compared to group NC, ^# for groups VDAS compared to group DAS). (d) The expressions of JAK2, STAT3, cyclinD1, and Bcl-2 were analyzed by western blot ( for group DM and group DAS compared to group NC, ^# for groups VDAS compared to group DAS).

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