Isorhapontigenin Modulates SOX9/TOLLIP Expression to Attenuate Cell Apoptosis and Oxidative Stress in Paraquat-Induced Acute Kidney Injury

Figure 2

Isorhapontigenin (ISO) ameliorates the paraquat- (PQ-) induced oxidative stress in NRK-52E cells. NRK-52E cells were pretreated with ISO (5 μM or 10 μM) for 30 min followed by a 24 h coincubation with 300 μM PQ. (a) The malondialdehyde (MDA) contents, (b) superoxide dismutase (SOD) activity, and (c) lactate dehydrogenase (LDH) leakage were determined using the commercially available kits. (d, e) Flow cytometry was performed to determine the reactive oxygen species (ROS) production in cells. The fluorescence intensity of 2,7-dichlorofluorescein (DCF) indicated ROS levels. (f) Protein levels of nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) were detected via Western blot. (g) The mRNA levels of HO-1 were measured via quantitative real-time PCR. Error bars represent standard deviations. values < 0.01; values < 0.001.

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