Isorhapontigenin Modulates SOX9/TOLLIP Expression to Attenuate Cell Apoptosis and Oxidative Stress in Paraquat-Induced Acute Kidney Injury

Figure 6

Isorhapontigenin (ISO) alleviates cell apoptosis and oxidative stress in the renal cortex of paraquat- (PQ-) acute kidney injury (AKI) rats. (a) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining showed the apoptotic cells in the renal cortex. Scale bars represent 200 μm or 50 μm; 100x or 400x magnification. (b) Expression of cell apoptosis-related proteins in the renal cortex was detected using Western blot. (c) Malondialdehyde (MDA) content in the renal cortex was measured using the commercially available kit. (d) Protein levels of nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) were detected using Western blot. (e) The mRNA levels of HO-1 were measured using quantitative real-time PCR (qPCR). Eight rats in each group. Error bars represent standard deviations. values < 0.001.

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