Modulating Oxidative Stress in B Cells Promotes Immunotherapy in Food Allergy

Figure 6

FGN promotes SIT efficacy in suppressing FA response in mice. A murine FA model was developed. FA mice were treated with the procedures as denoted in each panel. (a) Number of mice with diarrhea. (b) Core temperature (Temp) changes in mice recorded 30 min after the last antigen challenge. (c) Serum-specific IgE (sIgE) levels. (d–f) MCP1, EPX, and IL-4 levels in GLF (gut lavage fluids). (g, h) LPMCs were prepared and analyzed by FACS. (g) Gated plots show Breg counts. (h) Boxplots show Breg frequency in LPMCs. Data of boxplots are presented as median (IQR). , , and (ANOVA followed by Dunnett’s test), compared with the FA group. ^#, ^##, and ^### (ANOVA+Dunnett’s test), compared with the SIT group. Each group consists of 6 mice. Each bubble in boxplots presents data obtained from one mouse. NC: naïve control mice; FA: FA mice; saline: FA mice were treated with saline; SIT (FGN, SIT, and FGN): mice were treated with SIT (or FGN or FGN+SIT); Ab: mice were treated with anti-CD20 Ab to deplete B cells.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)