Cardiac Fibroblasts Promote Ferroptosis in Atrial Fibrillation by Secreting Exo-miR-23a-3p Targeting SLC7A11

Figure 7

miR-23a-3p accelerates ferroptosis in h9c2 cells via SLC7A11 downregulation. (a) RT-PCR analysis of miR-23 in h9c2 cells transfected with miR-23a-3p inhibitor and miR-23-3p mimics, respectively. (b) RT-PCR analysis of SLC7A11 expression normalized with GAPDH after transfected with mimics and inhibitor-miR-23a-3p, respectively. (c) Total iron level in h9c2 cells transfected with mimics-miR-23a-3p. (d) MDA level in h9c2 cells transfected with mimics-miR-23a-3p. (e, f) Mitochondrial membrane potential was detected through flow cytometry in h9c2 cells transfected with mimics-miR-23a-3p and treated with or without Fer-1. (g, h) Representative gel bands depicting FTH1, GPX4, and SLC7A11 proteins expression in h9c2 cells transfected with mimics-miR-23a-3p and treated with or without Fer-1. Data are presented as the mean ± SD,  =3. Statistical significance was determined using one-way ANOVA with a post hoc Dunnett test. , , and indicate  <0.05, 0.01, and 0.001, respectively. Abbreviations: mimics: h9c2 cells transfected with mimics-miR-23a-3p (25 μM) for 48 h; inhibitor: h9c2 cells transfected with inhibitor-miR-23a-3p (30 μM) for 48 h.

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