Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway

Figure 4

Autophagy flux changes mediated neurons degenerated and apoptosis after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. . (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). . (e) TEM images of neurons located in SVZ showed microstructure and edema. . Results are presented as , versus sham group, ^## and ^# ICH-IVH group versus MCC950 group.

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