Inhibition of PLK3 Attenuates Tubular Epithelial Cell Apoptosis after Renal Ischemia–Reperfusion Injury by Blocking the ATM/P53-Mediated DNA Damage Response

Figure 2

PLK3 expression was upregulated in TECs exposed to H/R injury and kidneys exposed to I/R injury. (a) PLK3 mRNA levels in HK-2 cells were measured by real-time RT–PCR after hypoxia and reoxygenation for different time periods. (b) PLK3 protein levels were measured by Western blot after different hypoxia time periods and the same reoxygenation time period. (c) PLK3 protein levels after the same hypoxia time period and different reoxygenation time periods. (d) PLK3 mRNA levels in the kidneys of C57BL/6J mice exposed to I/R were examined after 30 min of ischemia and different reperfusion times (6 h, 12 h, and 24 h). (e) PLK3 protein levels after different reperfusion times were observed by immunofluorescence (; magnification, 400x). (f) PLK3 protein levels were measured after different reperfusion times. (g-h) Creatinine and BUN levels after different reperfusion times. The data are presented as the . . , , , and . BUN: blood urea nitrogen; H24R3: hypoxia for 24 h and reperfusion for 3 h; Rep24: ischemia for 30 min and reperfusion for 24 h.

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