Coenzyme Q₀ Inhibits NLRP3 Inflammasome Activation through Mitophagy Induction in LPS/ATP-Stimulated Macrophages

Figure 2

CoQ₀ inhibits NLRP3 inflammasome activation in LPS/ATP-stimulated RAW264.7 macrophages. Cells were first treated with CoQ₀ (2.5-10 μM) and later stimulated with LPS (1 μg/mL) for 5 h followed by ATP (5 mM) treatment for 1 h. (a) The expression of NLRP3 and procaspase-1 protein was determined by Western blotting. (b) Caspase-1 activity was measured using a caspase-1 activity assay kit. (c) Pro-IL1β expressions in both lysis and supernatant and mature-IL1β in the supernatant were determined by Western blot using β-actin as internal control as well as band intensities were calculated by AlphaEaseFCTM (Genetic Technologies, Inc., Florida, USA) software. The data were calculated from the (SD) of three independent experiments and , compared with untreated control cells, and ^### compared with LPS/ATP-stimulated cells which was significant.

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