Reactive Oxygen Species are Essential for Placental Angiogenesis During Early Gestation

Figure 4

Overexpression of Flt-1 induces a PE-like phenotype in mice. (a) Mating and treatment plan for the establishment of the PE mouse model. (b, c) Systolic and diastolic BP of pregnant mice (b) and nonpregnant mice (c) measured using a tail vein cuff. (d) PAS staining of the kidney and quantification of the glomerulus open capillary area in different groups (mice per group); scale bars: 50 μm. (e) Fetal birth weight. NS group: pups from 4 dams (50/4), NC group: , mFlt-1: . F. Serum sFlt-1 levels in the NS, NC, and mFlt-1 groups on GD 11.5, as measured using ELISA. mice per group. (g) IHC staining for 4HNE (upper panel) and MDA (lower panel) in the mouse placenta on GD 18.5 in the NS and mFlt-1 groups. Scale bars: upper panel -1 mm and lower panel -100 μm. Data are presented as the . Data were analyzed using two-way (b, c) and one-way (d, e, f) ANOVA, followed by Sidak’s multiple comparison tests. , , and .

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