Research Article

Lipopolysaccharide Enhances Genotoxicity by Activating GADD45G and NF-κB in Human Corneal Epithelial Cells

Figure 3

Assessment of the mitochondrial membrane potential in HCEC cells treated with lipopolysaccharide (LPS). Fluorescence microscopy and flow cytometry were used to analyze the staining of HCEC cells with JC-1 dye. Incubation of LPS (1, 5, and 10 μg/mL) for 24 hours (a)–(d) shows bright green fluorescent staining in the HCEC cells. A FACS analysis shows the percentage of positive cells in the LPS-treated HCECs (e)–(h). Based on three cultures replicated for each experimental points (), the data represent (S.E.).