Research Article

Lipopolysaccharide Enhances Genotoxicity by Activating GADD45G and NF-κB in Human Corneal Epithelial Cells

Figure 7

The expression of GADD45G, NF-κB, and H2AX by RT-PCR in HCEC cells treated with lipopolysaccharide (LPS). mRNA expression levels of HCEC cells treated with LPS at 0, 1, 2, 4, 8, and 12 h were quantified (a)–(d). Relative expression and density of the target gene were normalized to that of housekeeping gene GAPDH. Based on three cultures replicated for each experimental points (), the data represent (S.E.). values of 0.05, 0.01, and 0.001 representing , , and as compared to untreated control cells (NC) were calculated using Kruskal-Wallis and Dunn’s tests.
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