Lipopolysaccharide Enhances Genotoxicity by Activating GADD45G and NF-κB in Human Corneal Epithelial Cells

Figure 9

Analysis of growth arrest and DNA damage-inducible factor immunostaining in HCEC cells treated with lipopolysaccharide (1, 5, and 10 μg/mL). An immunohistochemistry stain of Gadd45γ showing light microscopic images (a)–(d). To count the Gadd45γ positive cells per HPF magnification, densitometry analysis was performed using Image-J software (e). Using immunohistochemistry stains, (f)–(i) show light microscopy images of phosphor-γH2AX. The (j) graph shows the densitometry analysis of cells containing positive γH2AX using Image-J software to quantify the number of positive cells. Based on three cultures replicated for each experimental points (), the data represent (S.E.). values of 0.05, 0.01, and 0.001 representing , , and as compared to untreated control cells (NC) were calculated using Kruskal-Wallis and Dunn’s tests. The images were all captured at a 20× magnification.