Protective Effects of Cannabidiol on Chemotherapy-Induced Oral Mucositis via the Nrf2/Keap1/ARE Signaling Pathways

Figure 4

CBD enhances HOK proliferative capacity and cell migration in in vitro OM models. (a) Cell viability of HOK cells exposed to different concentrations of CBD (0.1-10 μM) for 48 hrs was detected by CCK-8 assay. (b) HOK viability after 24 hrs and 48 hrs exposure to 5-FU (0.1-40 μg/mL). vs. vehicle group. (c) CCK-8 results showing the proliferation of HOK cells after exposure to 5-FU (10 μg/mL) and CBD (0.5, 2.5, and 5 μM) for 1, 3, and 5 days. (d) Flow cytometry results of HOK cells treated with 5-FU and CBD to detect the apoptosis rates. (e) mRNA expression levels of Bax and Bcl-2 in HOK cells as measured by qRT-PCR. β-Actin was used as the internal control. (f) Representative images of wound healing assays for HOK migration at 0 h, 12 hrs, and 24 hrs. Scale bar, 200 μm. (g) Quantitative analysis of the healing rates. Data are expressed as the . ^#vs. vehicle; vs. 5-FU alone group.

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