Protective Effects of Cannabidiol on Chemotherapy-Induced Oral Mucositis via the Nrf2/Keap1/ARE Signaling Pathways

<div>ML385 blunted the antioxidant effects of CBD in <i>in vitro</i> OM model. (a) HOK cells were pretreated with CBD for 12 hrs, and then, 5-FU and ML385 (10 <i>μ</i>M) were added and incubated for another 12 hrs. Western blotting of Nrf2, HO-1, and NQO1 protein levels was performed. <i>β</i>-Actin was used as the internal control. (b) Grayscale analysis of the blots. (c) HOK was harvested to measure the mRNA expression levels of <i>Nrf2</i>, <i>HO-1</i>, and <i>NQO1</i> using qRT-PCR. <i>β</i>-Actin was used as the internal control. (d) DCFH-DA staining of the treated HOK cells to evaluate intracellular ROS generation. Scale bar, 200 <i>μ</i>m. (e) Quantitative analysis of the mean fluorescence intensity in (d). 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238 395C212 395 165 379 127 364L153 550Z"></path></g></svg><i>vs.</i> the 5-FU alone group; <sup>#</sup><svg height="9.2729pt" id="M38" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 44.8393 9.2729" width="44.8393pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"></path></g><g transform="matrix(.013,0,0,-0.013,22.921,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 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Oxidative Medicine and Cellular Longevity

fig7

Figure 7

Figure 7: Protective Effects of Cannabidiol on Chemotherapy-Induced Oral Mucositis via the Nrf2/Keap1/ARE Signaling Pathways 