Research Article
[Retracted] Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway
Figure 4
Metformin alleviates H2O2-induced ROS generation and DNA damage in ADSCs. (a) The cellular ROS was detected by the probe DCFH-DA under a fluorescence microscope. Scale μm. (b) Representative analysis of ROS levels in ADSCs by flow cytometry (left). The levels of intracellular ROS in ADSCs detected by the DCF-MFI (right). The results are presented as (). Using one-way ANOVA, vs. control group and # and ## vs. H2O2 group. (c) Representative immunofluorescence staining for γH2A.X. Scale μm. (d) Representative western blots show protein levels of Rad51 and γH2A.X. The results are presented as (). Using one-way ANOVA, ∗∗ and vs. control group and # vs. H2O2 group.
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