Research Article

Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4–PGC-1α–PPARα/PPARγ Signaling in Isolated Beating Rat Atria

Figure 4

Effects of p38 MAPK and Akt on ANP secretion and dynamics induced by CCK-8s in isolated beating rat atria. (a) The protein expression levels of p-p38 MAPK and p-Akt were detected by Western blot. (b) The statistics histograms of Western blot were expressed as band density normalized versus t-p38. (c) The statistics histograms of Western blot were expressed as band density normalized versus t-Akt. (d, f) Atrial ANP secretion by radioimmunoassay. (e, g) Atrial pulse pressure by RM6240BD. Cont: control; CCK-8s: sulfated CCK-8; GLX: GLX351322, an inhibitor of NOX4; SB: SB239063, an antagonist of p38 MAPK; LY: LY294002, an inhibitor of Akt. Data were expressed as . (a–c) ; (d–g) . , , and vs. control period or group; #, ##, and ### vs. CCK-8s period or group.
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