Research Article

Formononetin Inhibits Hepatic I/R-Induced Injury through Regulating PHB2/PINK1/Parkin Pathway

Figure 4

Effects of FN on the Parkin/PINK1 regulating mitophagy. (a) The protein expression levels of Parkin, PINK1, LC3 II, p62, and Beclin1 were measured by western blotting. (b) The mRNA expression levels of Parkin, PINK1, LC3 II, p62, and Beclin1 were measured by RT-PCR. (c) 3-Methyladenine (3-MA), a widely used inhibitor of autophagy, was used together with FN, and Masson staining was used to evaluate the liver injury (60×). (d) The protein expression levels of LC3II, P62, and Beclin1 were measured after AAV-PINK1 treatment. (e) Representative pictures of TME; autolysosome was marked as ASS. ## vs. control group, vs. model group, and && vs. FN group. in every group.
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