Aβ1–40 Oligomers Trigger Neutrophil Extracellular Trap Formation through TLR4- and NADPH Oxidase-Dependent Pathways in Age-Related Macular Degeneration

Figure 5

Aβ1–40 oligomers induce NETs formation via NOX. (a) Neutrophils were incubated with PMA (200 nM), Aβ1–40 oligomers (10 μM), or the control for 4 h at 37°C. DAPI was used to label extracellular DNA. H3-cit was immunolabelled with an antibody in the absence or presence of the NOX inhibitor DPI (10 μM). (b). (c) Measurements of extracellular DNA and NE levels indicated the presence of NETs after the treatment described above. (d) Western blot analysis of H3-cit, NOX2, and NOX4 expression in neutrophils following the treatment described above. , , .

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